公开(公告)号：US20230094152A1

公开(公告)日：2023-03-30

申请号：US17936286

申请日：2022-09-28

Applicant: California Institute of Technology

Inventor： Shirin Shivaei ,  Mikhail G. Shapiro

IPC: C12N15/86 ,  C12N15/63 ,  A61B8/08

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in imaging of in situ gene expression. There are provided, in some embodiments, viral vector compositions. Disclosed herein includes a single viral vector comprising one or more gas vesicle assembly (GVA) gene(s) encoding one or more GVA protein(s), and one or more gas vesicle structural (GVS) gene(s) encoding one or more GVS protein(s). The one or more GVA protein(s) and the one or more GVS protein(s) can be capable of forming gas vesicles (GVs) upon expression in a cell.