公开(公告)号：US20240402142A1

公开(公告)日：2024-12-05

申请号：US18748622

申请日：2024-06-20

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Klaus Gebauer ,  Dan Hermansson

IPC: G01N30/60 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56

Abstract: A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube having a closed first end comprising an inlet/outlet, a media inlet adjacent a second end of the column tube and an adaptor positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

公开(公告)号：US12044663B2

公开(公告)日：2024-07-23

申请号：US16067628

申请日：2017-01-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer ,  Dan Hermansson

IPC: G01N30/60 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56

CPC classification number: G01N30/6004 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N2030/565 ,  G01N30/6021 ,  G01N30/606

Abstract: A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.