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公开(公告)号：US20230059192A1
公开(公告)日：2023-02-23
申请号：US17821018
申请日：2022-08-19
发明人： Luke J. Turechek
IPC分类号： C12Q1/6855 , C12N15/10 , C12N15/113
摘要： The present disclosure provides methods for determining oligonucleotide purity and/or characterizing small RNAs. The methods comprising ligating adapters comprising unique molecule identifiers (UMIs), amplifying ligation products to generate a library, and sequencing the library. The methods of the disclosure exhibit reduced or no bias in terms of discrepancies that can arise during the ligation and/or amplification steps of the methods.
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