公开(公告)号：US20040071690A1

公开(公告)日：2004-04-15

申请号：US10367956

申请日：2003-02-19

申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANIZATION

发明人： Peter John Hudson ,  Alex Andrew Kortt ,  Robert Alexander Irving ,  John Leslie Atwell

IPC分类号： A61K039/395 ,  C07K016/18

CPC分类号： C07K16/4208 ,  A61K38/00 ,  A61K47/6425 ,  A61K47/6803 ,  A61K47/6817 ,  A61K47/6879 ,  A61K51/109 ,  C07K14/70521 ,  C07K14/70532 ,  C07K16/00 ,  C07K16/40 ,  C07K2317/622 ,  C07K2319/00

摘要： This invention provides polyvalent or polyspecific protein complexes, comprising three or more polypeptides which associate to form three or more functional target-binding regions (TBRs), and in which each individual polypeptide comprises two or more immunoglobulin-like domains which are covalently joined together, such that two Ig-like domains in a single polypeptide do not associate with each other to form a TBR. By using a linker peptide of fewer than three amino acid residues the immunoglobulin-like domains of the individual polypeptides are prevented from associating, so that complex formation between polypeptides is favoured. Preferably the polyvalent or polyspecific protein is a trimer or tetramer. The proteins of the invention have specificities which may be the same or different, and are suitable for use as therapeutic, diagnostic or imaging agents.

摘要翻译： 本发明提供了多价或多特异性蛋白复合物，其包含三个或更多个缔合以形成三个或更多个功能性靶结合区（TBR）的多肽，并且其中每个单个多肽包含共价连接在一起的两个或更多个免疫球蛋白样结构域， 使得单个多肽中的两个Ig样结构域不相互结合以形成TBR。 通过使用少于三个氨基酸残基的接头肽，可以防止单个多肽的免疫球蛋白样结构域缔合，从而有利于多肽之间的复合物形成。 优选地，多价或多特异性蛋白质是三聚体或四聚体。 本发明的蛋白质具有相同或不同的特异性，并且适合用作治疗，诊断或成像剂。