公开(公告)号：US3761586A

公开(公告)日：1973-09-25

申请号：US3761586D

申请日：1971-03-02

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： MATSUMOTO Y ,  KOYAMA K ,  TAKEDA Y

IPC分类号： A61K35/58 ,  A61K17/00

CPC分类号： A61K35/58

摘要： The hemostatic agent of the present invention is obtained by the following process: An aqueous solution of Trimeresurus okinavensis venom is treated with almost the same volume of an organic solvent such as chloroform, ether and ethyl acetate to remove lipids, and the water layer is collected and dialysed against a distilled water. Dyalysate is passed through dextran gel column, said dextran gel having a molecular weight fractionation range of 1,000 to 50,000, to adsorb thereon and eluted with phosphate buffered solution (pH 7 - 8). The fraction having strong coagulating activity is collected in the recalcification test and passed through a column filled with a basic ion exchange resin to allow the active substance to adsorb thereon and eluted with ethyl acetate (pH about 7). The fraction having the highest blood coagulating activity is collected in the recalcification test, and lyophilised.

摘要翻译： 本发明的止血剂通过以下方法得到：用几乎相同体积的有机溶剂如氯仿，乙醚和乙酸乙酯处理Trimeresurus okinavensis毒液的水溶液以除去脂质，并收集水层 并用蒸馏水透析。 透明质酸钠通过葡聚糖凝胶柱，所述葡聚糖凝胶具有1,000至50,000的分子量分级范围，以吸附并用磷酸盐缓冲溶液（pH7-8）洗脱。 在再钙化试验中收集具有强凝结活性的级分，并通过填充有碱性离子交换树脂的柱，使活性物质吸附在其上并用乙酸乙酯（pH约7）洗脱。 在再钙化试验中收集具有最高凝血活性的级分，并冻干。