公开(公告)号：US20160185817A1

公开(公告)日：2016-06-30

申请号：US14910555

申请日：2014-08-08

Applicant: Biogen MA Inc.

Inventor： Lily ZHU ,  Anxhela KOLE ,  John KULMAN ,  Marisol ACOSTA

IPC: C07K1/36 ,  C07K14/755

CPC classification number: C07K1/36 ,  C07K14/755 ,  C07K2319/31

Abstract: The invention is directed to methods of purifying a chimeric protein comprising subjecting the chimeric protein to a factor VIII-specific affinity chromatography, and subjecting the chimeric protein to an AEX chromatography; wherein the chimeric protein comprises a factor VIII protein or a fragment thereof. The chimeric protein purified by the present methods shows improved factor VIII activity.

Abstract translation: 本发明涉及纯化嵌合蛋白的方法，包括使嵌合蛋白进行因子VIII特异性亲和层析，并使嵌合蛋白进行AEX色谱; 其中所述嵌合蛋白包含因子VIII蛋白或其片段。 通过本方法纯化的嵌合蛋白显示出改善的因子VIII活性。