公开(公告)号：US20140162266A1

公开(公告)日：2014-06-12

申请号：US14097923

申请日：2013-12-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Niels Klitgord ,  John Regan ,  Svilen Tzonev ,  Eli Hefner ,  Jennifer R. Berman

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q2537/16 ,  C12Q2563/159

Abstract: This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.

Abstract translation: 本公开提供了用于测量高度扩增和/或丰富的基因组基因座的拷贝数的方法。 本文认识到需要用于确定核酸拷贝数的方法，特别是在一个待定量的位点（即目标）相对于已知丰度的基因座（即参考文献）相对更丰富的情况下。 在一些情况下，该方法包括将查询核酸样品与稀释的核酸样品组合，并测量与组合样品中的参考序列相比的靶序列的相对拷贝数。