公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

Applicant: Berkeley Lights, Inc.

Inventor： Matthew Asuka Kubit ,  Joshua David Mast ,  John Junyeon Kim ,  Alexander Gerald Olson ,  Preston Lock Ng ,  Arlvin Louis Ellefson ,  Shruthi Sreedhar Kubatur ,  Vincent Haw Tien Pai ,  Minha Park ,  Po-Yuan Tung ,  Jason C. Briggs ,  Patrick N. Ingram ,  Katrine Elise Dailey ,  Maryam Shansab ,  Jason M. McEwen ,  Adrienne T. Higa ,  Hongye Zhou ,  Zhen Hu ,  John A. Tenney

IPC: C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6804

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q2600/16

Abstract: Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.