公开(公告)号：US11041154B2

公开(公告)日：2021-06-22

申请号：US16632792

申请日：2017-09-29

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Samira Kiani ,  Xiao Wang ,  David Menn ,  Mo Reza Ebrahimkhani

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/63

Abstract: Provided herein are tools for understanding and engineering dynamics of synthetic genetic circuits which utilize CRISPR components. More particularly, methods, systems, and compositions for directing Cas9 activity using a fluorescent guide RNA (fgR-NA) which fluoresces in the presence of small molecules (e.g., DFHBI-IT) are described and illustrated in the present provisional application.