公开(公告)号：US12216053B1

公开(公告)日：2025-02-04

申请号：US17354710

申请日：2021-06-22

Applicant: Amazon Technologies, Inc.

Inventor： Miro Yakov Shverdin ,  Annapurna Karicherla ,  Carla Alejandra Gimenez

IPC: G01N1/00 ,  C12Q1/70 ,  G01N21/64

Abstract: Systems and methods for testing a biological sample for each of two or more target pathogens employ fluorescence detection from a single fluorescence reading chamber. A method of testing a biological sample for each of two or more target pathogens includes forming a detection cycle liquid by combining detection cycle compounds with a biological sample liquid formed from a biological sample. The detection cycle compounds include respective CRISPR enzymes for each of the two or more target pathogens. The respective CRISPR enzymes are tagged with respective spectrally unique fluorescent probes. The detection cycle liquid is exposed to a multi-wavelength fluorescence excitation light. A multi-wavelength fluorescence light emission detection signal indicative of intensity of fluorescence light emitted by the detection cycle liquid over multiple wavelengths is processed to generate detection data indicative of presence or absence of each of the two or more target pathogens in the biological sample.