公开(公告)号：US09890417B2

公开(公告)日：2018-02-13

申请号：US14531563

申请日：2014-11-03

Applicant: Agilent Technologies, Inc.

Inventor： Kristin Bernick ,  Robert Ach ,  Mistuni Ghosh ,  Brian Smart

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2563/125 ,  C12Q2563/131

Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

公开(公告)号：US20160122800A1

公开(公告)日：2016-05-05

申请号：US14531563

申请日：2014-11-03

Applicant: Agilent Technologies, Inc.

Inventor： Kristin Bernick ,  Robert Ach ,  Mistuni Ghosh ,  Brian Smart

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2563/125 ,  C12Q2563/131

Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

Abstract translation: 除此之外，本公开提供了检测靶核酸的方法。 该方法的方面包括：（a）获得与靶核酸互补的标记核酸探针，其中所述探针包含捕获标签; （b）将位于固定细胞中的探针与靶核酸原位杂交以产生双链体; （c）通过捕获标签将双链体中的探针与过氧化物酶缀合物连接以产生过氧化物酶标记的双链体; 和（d）过氧化物酶标记的双链体与过氧化物酶底物孵育，其中过氧化物酶缀合物的过氧化物酶活性催化底物在双相体附近的沉积，从而产生可检测的信号。