公开(公告)号：US09193992B2

公开(公告)日：2015-11-24

申请号：US13894886

申请日：2013-05-15

Applicant: Agilent Technologies, Inc.

Inventor： Bo U. Curry ,  Jayati Ghosh ,  Brian Jon Peter ,  Arjun Vadapalli

IPC: C12Q1/68 ,  C07H21/00 ,  G06F19/18

CPC classification number: C12Q1/6827 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  G06F19/18

Abstract: A method for determining the ploidy of a test genome is provided. In some embodiments, the method may comprises: a) obtaining a plurality of ratios for polymorphisms that are distributed throughout a test genome, wherein each of the ratios is a ratio of the measured copy number of uncut allele in a polymorphic site relative to the measured copy number of the uncut allele in the reference sample; b) taking the log of the ratios and plotting a distribution of the reference corrected log ratios of the SNP probes; and c) determining the ploidy of said the genome based on the number of peaks in that distribution.

Abstract translation: 提供了测定基因组倍性的方法。 在一些实施方案中，所述方法可以包括：a）获得分布在整个测试基因组中的多态性的多个比率，其中每个比率是多态性位点中未切割等位基因的测定拷贝数相对于测量的 参考样品中未切割等位基因的拷贝数; b）记录比率对数，并绘制SNP探针的参考校正对数比的分布; 和c）基于该分布中的峰的数目确定所述基因组的倍性。