公开(公告)号：US20220184156A1

公开(公告)日：2022-06-16

申请号：US17547323

申请日：2021-12-10

Applicant: ASTRAZENECA UK LIMITED

Inventor： JINLIN JIANG ,  NICOLE BLECKWENN ,  RAGHAVAN VENKAT ,  DANIEL PAPPAS ,  BENJAMIN RUSH ,  GEORGE CHACKO

IPC: A61K35/761 ,  C12N7/00 ,  A61K39/235

Abstract: Methods for the production of adenoviruses which are suitable for use in a vaccine, and methods for increasing the yield of adenoviruses during production. These methods include adding an adenovirus to a cell population in culture; culturing the cell population under conditions which are permissive for infection of the cell population with the adenovirus to provide a cell population comprising adenovirus-infected cells; culturing the cell population comprising adenovirus-infected cells under conditions which are permissive for replication of the adenovirus; and harvesting the adenovirus from the culture.

公开(公告)号：US20240035003A1

公开(公告)日：2024-02-01

申请号：US18256484

申请日：2021-12-10

Applicant: ASTRAZENECA UK LIMITED

Inventor： JINLIN JIANG ,  NICOLE BLECKWENN ,  RAGHAVAN VENKAT ,  DANIEL PAPPAS ,  BENJAMIN RUSH ,  GEORGE CHACKO

IPC: C12N7/02 ,  C12N15/86 ,  A61K39/215

CPC classification number: C12N7/02 ,  C12N15/86 ,  A61K39/215 ,  A61K2039/5254

Abstract: Methods for the production of adenoviruses which are suitable for use in a vaccine, and methods for increasing the yield of adenoviruses during production. These methods include adding an adenovirus to a cell population in culture; culturing the cell population under conditions which are permissive for infection of the cell population with the adenovirus to provide a cell population comprising adenovirus-infected cells; culturing the cell population comprising adenovirus-infected cells under conditions which are permissive for replication of the adenovirus; and harvesting the adenovirus from the culture.