公开(公告)号：US20210198693A1

公开(公告)日：2021-07-01

申请号：US17190745

申请日：2021-03-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Hugh Mason ,  Mary Pardhe ,  Andrew Diamos

IPC: C12N15/86 ,  C12N15/82

Abstract: Improved plant transient expression systems using optimized geminiviral vectors that efficiently produce heteromultimeric proteins are described herein. Examples of high yields are shown herein, including two, three, or four fluorescent proteins coexpressed simultaneously. Various antibodies were produced using the optimized vectors with special focus given to the creation and production of a chimeric broadly neutralizing anti-flavivirus antibody. The variable regions of this murine antibody, 2A10G6, were codon optimized and fused to a human IgG1. Analysis of the chimeric antibody showed that it was efficiently expressed in plants, can be purified to near homogeneity by a simple one-step purification process, retains its ability to recognize the Zika virus envelope protein, and induce an immune response against Zika virus. Two other monoclonal antibodies were produced at similar levels. This technology is versatile tool for the production of a wide spectrum of pharmaceutical multi-protein complexes in a fast, powerful, and cost-effective way.

公开(公告)号：US11058766B2

公开(公告)日：2021-07-13

申请号：US16404698

申请日：2019-05-06

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Hugh Mason ,  Andrew Diamos ,  Mary Pardhe ,  Brandon Favre

IPC: A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  C07K14/005 ,  A61K39/295 ,  A61P31/20 ,  C12N7/00 ,  A61K39/145

Abstract: The disclosure relates to vaccination compositions, for example, against human papillomavirus, Zika virus, and flu virus. The disclosure also relates to vectors for producing the virus-like particles and immune complex platforms of the vaccination compositions.

公开(公告)号：US12297443B2

公开(公告)日：2025-05-13

申请号：US17190745

申请日：2021-03-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Hugh Mason ,  Mary Pardhe ,  Andrew Diamos

IPC: C12N15/86 ,  C12N15/82

Abstract: Improved plant transient expression systems using optimized geminiviral vectors that efficiently produce heteromultimeric proteins are described herein. Examples of high yields are shown herein, including two, three, or four fluorescent proteins coexpressed simultaneously. Various antibodies were produced using the optimized vectors with special focus given to the creation and production of a chimeric broadly neutralizing anti-flavivirus antibody. The variable regions of this murine antibody, 2A10G6, were codon optimized and fused to a human IgG1. Analysis of the chimeric antibody showed that it was efficiently expressed in plants, can be purified to near homogeneity by a simple one-step purification process, retains its ability to recognize the Zika virus envelope protein, and induce an immune response against Zika virus. Two other monoclonal antibodies were produced at similar levels. This technology is versatile tool for the production of a wide spectrum of pharmaceutical multi-protein complexes in a fast, powerful, and cost-effective way.

公开(公告)号：US20190336596A1

公开(公告)日：2019-11-07

申请号：US16404698

申请日：2019-05-06

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Hugh Mason ,  Andrew Diamos ,  Mary Pardhe

IPC: A61K39/29 ,  A61K39/12 ,  A61P31/20 ,  C12N7/00 ,  A61K39/145

Abstract: The disclosure relates to vaccination compositions, for example, against human papillomavirus, Zika virus, and flu virus. The disclosure also relates to vectors for producing the virus-like particles and immune complex platforms of the vaccination compositions.