公开(公告)号：US20240262908A1

公开(公告)日：2024-08-08

申请号：US18429717

申请日：2024-02-01

Applicant: AMGEN INC.

Inventor： Gang Xiao ,  Hyo Seon Chung ,  Quanzhou Luo ,  Tamer Eris ,  Joseph Edward Basconi ,  Xiaoyang Zhao ,  Pavel Bondarenko ,  Krista Marie Petty ,  Oliver Kaltenbrunner

IPC: C07K16/28 ,  C12N5/071 ,  C12N9/64

CPC classification number: C07K16/2803 ,  C12N5/0682 ,  C12N9/641 ,  C07K2317/522 ,  C12N2500/02 ,  C12N2501/999 ,  C12N2523/00 ,  C12Y304/22034

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

公开(公告)号：US20150004646A1

公开(公告)日：2015-01-01

申请号：US14365496

申请日：2012-12-14

Applicant: Amgen Inc.

Inventor： Thomas M. McNerney ,  Krista Petty ,  Anne C Thomas ,  Xiaoyang Zhao

IPC: C12P21/00

CPC classification number: C07K1/145 ,  C07K16/00 ,  C12P21/00 ,  C12P21/02

Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

Abstract translation: 本发明涉及从哺乳动物细胞培养液中收获重组蛋白的方法。 该方法利用阳离子聚合物，非离子聚合物和非离子表面活性剂。

公开(公告)号：US10023608B1

公开(公告)日：2018-07-17

申请号：US14208043

申请日：2014-03-13

Applicant: AMGEN INC.

Inventor： Junfen Ma ,  Xiaoyang Zhao ,  Brian Williamson

IPC: C07K1/20 ,  C07K16/24 ,  C07K19/00 ,  C07K1/16

Abstract: Disclosed are methods of purifying a protein sample. More specifically, provide are methods of removing or reducing the amount of high molecular weight species and/or high mannose species from a protein sample using a mixed mode chromatography step and a hydrophobic interaction chromatography step.

公开(公告)号：US09371554B2

公开(公告)日：2016-06-21

申请号：US14365496

申请日：2012-12-14

Applicant: Amgen Inc.

Inventor： Thomas M McNerney ,  Krista Petty ,  Anne C Thomas ,  Xiaoyang Zhao

IPC: C12P21/00 ,  C12P21/02 ,  C07K1/14

CPC classification number: C07K1/145 ,  C07K16/00 ,  C12P21/00 ,  C12P21/02

Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

Abstract translation: 本发明涉及从哺乳动物细胞培养液中收获重组蛋白的方法。 该方法利用阳离子聚合物，非离子聚合物和非离子表面活性剂。