公开(公告)号：US20180105589A1

公开(公告)日：2018-04-19

申请号：US15696138

申请日：2017-09-05

Applicant: AMGEN INC.

Inventor： Thomas M. DILLON ,  Pavel BONDARENKO ,  Jette WYPYCH ,  Martin J. ALLEN ,  Alain BALLAND ,  Margaret Speed RICCI ,  Amy GUO ,  Wenyan SHEN ,  Jeonghoon SUN ,  Christopher J. VEZINA

IPC: C07K16/24 ,  C07K16/28 ,  C07K16/00

CPC classification number: C07K16/245 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2875 ,  C07K2317/53 ,  C07K2317/56

Abstract: The present invention is generally directed to methods of producing an increase in the enrichment and/or recovery of preferred forms of monoclonal antibodies. More particularly, the invention relates to methods for eliminating disulfide heterogeneity in the hinge region of recombinant IgG2 antibody proteins.

公开(公告)号：US20230349912A1

公开(公告)日：2023-11-02

申请号：US18026678

申请日：2021-09-17

Applicant: AMGEN INC.

Inventor： Gang XIAO ,  Pavel BONDARENKO ,  Liuqing SHI ,  Thomas M. DILLON ,  Da REN ,  Margaret Speed RICCI ,  Weidong CUI ,  John Joseph HARRAHY ,  Jake PAWLOWSKI ,  Andrew DYKSTRA ,  Dylan RIGGS

IPC: G01N33/68

CPC classification number: G01N33/6821 ,  G01N33/6848 ,  G01N2333/976

Abstract: Provided herein are methods of processing a polypeptide or protein for analysis, e.g., peptide mapping analysis by mass spectrometry. In exemplary embodiments, the method comprises incubating a digested sample at a mildly acidic pH and/or in the presence of a chaotrope, wherein the digested sample is produced by digesting a polypeptide with a protease to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with a protease which cleaves C-terminal to tryptophan to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with trypsin at an enzyme:substrate (E:S) weight ratio of about 1:1 to about 1:15 to produce a digested sample comprising at least two peptides. In exemplary aspects, the digested sample comprises at least one or two peptides each comprising a tyrosine at the C-terminus.