公开(公告)号：US20240425787A1

公开(公告)日：2024-12-26

申请号：US18701341

申请日：2022-10-21

Applicant: ALPVISION S.A. ,  FINALSPARK SARL

Inventor： Jean-Marc COMBY ,  Flavio MOR ,  Martin KUTTER ,  Frédéric JORDAN

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/36 ,  C12M3/00

Abstract: A microfluidics system to maintain alive and control cell cultures such as neuronal cell cultures over an air-liquid interface is arranged to prevent formation of air bubbles and liquid overflows in fully automated 24/7 operation over the mid-long term. A pumping device pushes a liquid through an inlet of the microfluidics channel, then through an area of the microfluidics channel that is under the air-liquid interface, then through an outlet of the microfluidics channel, assisted with geometrical arrangements of the microfluidics channel outlet and optionally inlet placements relative to the porous membranes of the air-liquid interface. The pumping device is programmed with different flow rates, flow directions, and flow durations according to cell culture features and events detected with a computer vision system and/or an electrophysiological signal processing system to automatically adapt the parameters of the pumping device to the current state of the cell culture over the air-liquid interface.