公开(公告)号：US20150307628A1

公开(公告)日：2015-10-29

申请号：US14647480

申请日：2013-11-27

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Yong Sung KIM ,  Hye Ji CHOI ,  Eun Sil SUNG

IPC: C07K16/46

CPC classification number: C07K16/46 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/2878 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/526 ,  C07K2317/622 ,  C07K2319/30

Abstract: Disclosed are a CH3 domain variant pair of an antibody, a method for preparing same, and a use thereof. A mutation is induced in the CH3 domain so as to improve a yield of forming a heterodimer heavy chain constant region of an antibody. The CH3 domain heterodimer forms a heterodimer heavy chain constant region with a high efficiency of 90 to 95% or more and also has outstanding heat stability. A heterodimer heavy chain constant region including the CH3 domain heterodimer can construct a bispecific monoclonal antibody which simultaneously recognizes two kinds of antigens. The CH3 domain heterodimer and the bispecific antibody or fusion protein of an antibody constant region comprising same can be usefully applied to the treatment or prevention of a disease associated with a target antigen or a target protein.

Abstract translation: 公开了抗体的CH3结构域变体对，其制备方法及其用途。 在CH3结构域中诱导突变，从而提高形成抗体的异源二聚体重链恒定区的产量。 CH3结构域异二聚体形成高效率为90〜95％以上的异源二聚体重链恒定区，热稳定性优异。 包含CH3结构域异源二聚体的异二聚体重链恒定区可以构建同时识别两种抗原的双特异性单克隆抗体。 包含其的抗体恒定区的CH3结构域异二聚体和双特异性抗体或融合蛋白可有效地应用于治疗或预防与靶抗原或靶蛋白相关的疾病。

公开(公告)号：US20180237541A1

公开(公告)日：2018-08-23

申请号：US15944505

申请日：2018-04-03

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Yong Sung KIM ,  Hye Ji CHOI ,  Eun Sil SUNG

IPC: C07K16/46 ,  C07K16/28 ,  C07K16/00

CPC classification number: C07K16/46 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/2878 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/526 ,  C07K2317/622 ,  C07K2319/30

Abstract: Disclosed are a CH3 domain variant pair of an antibody, a method for preparing same, and a use thereof. A mutation is induced in the CH3 domain so as to improve a yield of forming a heterodimer heavy chain constant region of an antibody. The CH3 domain heterodimer forms a heterodimer heavy chain constant region with a high efficiency of 90 to 95% or more and also has outstanding heat stability. A heterodimer heavy chain constant region including the CH3 domain heterodimer can construct a bispecific monoclonal antibody which simultaneously recognizes two kinds of antigens. The CH3 domain heterodimer and the bispecific antibody or fusion protein of an antibody constant region comprising same can be usefully applied to the treatment or prevention of a disease associated with a target antigen or a target protein.

公开(公告)号：US20180346600A1

公开(公告)日：2018-12-06

申请号：US15778966

申请日：2016-10-12

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Yong Sung KIM ,  Hye Ji CHOI ,  Ji Hee HA

IPC: C07K16/46 ,  C12N15/81 ,  C12N15/10

CPC classification number: A61K39/395 ,  C07K16/46 ,  C12N15/81

Abstract: A method for evaluating and screening a mutant inducing the high-efficiency formation of heterodimers from a human antibody heavy chain constant region mutant pair combination library in order to increase the yield of formation of human antibody heterodimeric heavy chain constant regions. A heterodimeric heavy chain constant region (heterodimeric F) library is obtained by the method. A CH3 domain mutant pair, in which the formation of the heterodimeric heavy chain constant regions is preferred in the library, forms heterodimeric heavy chain constant regions at a high yield of at least 80-90%, and also has excellent thermal stability and retains binding ability to the heavy chain constant region receptor (FcRn).