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公开(公告)号:US20050032213A1
公开(公告)日:2005-02-10
申请号:US10493523
申请日:2002-10-25
申请人: John Sinden , Ziping Dong
发明人: John Sinden , Ziping Dong
IPC分类号: C12N15/09 , A61K35/50 , A61K48/00 , A61P43/00 , C07K14/82 , C12N5/10 , C12N9/12 , C12N15/12 , C12N15/54 , C12N15/63 , C12N15/85
CPC分类号: C12N15/85 , A61K35/50 , C07K14/82 , C12N9/1205 , C12N2830/00 , C12N2830/15 , C12N2830/60 , C12N2830/85 , C12N2840/203
摘要: Recombinant vectors or constructs are prepared comprising a polynucleotide that encodes a conditionally-induced oncogene operably linked to the enhancer element of the second intron of the nestin gene, or a functional fragment thereof. The constructs can be used to produce conditionally immortal cells, for transplantation.
摘要翻译: 制备重组载体或构建体,其包含编码与巢蛋白基因的第二内含子的增强子元件可操作地连接的条件诱导的癌基因的多核苷酸或其功能性片段。 该构建体可用于产生条件性永生细胞用于移植。