公开(公告)号：US20240352442A1

公开(公告)日：2024-10-24

申请号：US18137786

申请日：2023-04-21

申请人： Zhejiang Difference Biological Technology Co., Ltd.

发明人： Shuihua MAO ,  Mengyun ZHOU ,  Ruiting CHEN ,  Xiaofei LIU ,  Jiasheng SONG

IPC分类号： C12N15/10 ,  C12N7/04

CPC分类号： C12N15/102 ,  C12N7/04 ,  C12N2760/16121

摘要： The present disclosure relates to a method for attenuating an influenza virus, an attenuated influenza virus strain and use thereof, and belongs to the technical field of attenuated vaccines. In the present disclosure, the method includes the following steps: conducting synonymous mutation on an overlap of an M2 gene and an M1 gene in an M gene of an influenza A virus, while ensuring integrity and invariance of an amino acid sequence encoded by the M1 gene; conducting mutation into termination codon and deletion of partial nucleotide sequence in a transmembrane domain of the M2 gene, and rescuing an attenuated influenza virus strain using a reverse genetic system.

公开(公告)号：US11510976B2

公开(公告)日：2022-11-29

申请号：US16766048

申请日：2018-06-01

申请人： Zhejiang Difference Biological Technology Co., Ltd.

发明人： Jiasheng Song

IPC分类号： A61K39/145 ,  C12N15/87 ,  A61K39/00

摘要： An H7 avian influenza vaccine strain which differentiates infected from vaccinated animals, a preparation method therefor, and an application. The highly pathogenic H7 avian influenza not only brings about huge economic losses to the livestock industry, but also seriously threatens public health safety. Conventional H7 avian influenza whole virus inactivated vaccines do have advantages such as being reliable in terms of effect, low in terms of cost and wide in terms of application range, but cannot serologically differentiate infected from vaccinated animals. The present invention uses NA of influenza B as a label to establish a method for constructing an H7 avian influenza vaccine strain which differentiates infection from vaccination, and may be used for the prevention, control and decontamination of the H7 avian influenza.

公开(公告)号：US11505786B2

公开(公告)日：2022-11-22

申请号：US15733120

申请日：2018-06-01

申请人： ZHEJIANG DIFFERENCE BIOLOGICAL TECHNOLOGY CO., LTD.

发明人： Jiasheng Song

IPC分类号： C12N7/00 ,  A61K39/145 ,  C07K14/005 ,  A61K39/00

摘要： Provided is an application of a label gene sequence in the preparation of an H9 avian influenza vaccine strain which differentiates influenza A virus infection from vaccination, the label gene sequence containing a DNA sequence for coding an influenza B virus NA protein extracellular region amino acid sequence. Also provided are an H9 avian influenza vaccine strain which differentiates influenza A virus infection from vaccination, a preparation method therefor, and an application.