公开(公告)号：US20240417773A1

公开(公告)日：2024-12-19

申请号：US18689866

申请日：2023-04-11

Applicant: Zhejiang Cancer Hospital

Inventor： Wenjie WU ,  Ming ZHAO ,  Jinhui CHANG ,  Ming LIU ,  Yixuan JIN ,  Ali LI ,  Jie LYV

IPC: C12Q1/22 ,  G01N21/64

Abstract: The present disclosure discloses a method for rapidly determining survival condition of spores. The method includes the following steps: S100: performing sterilization treatment on the spore; S200: preparing a spore suspension with the spore subjected to the sterilization treatment; S300: dripping an appropriate amount of the above prepared spore suspension onto a solid medium, wherein 4-MUG is contained in the solid medium; S400: observing the solid medium under an excitation light source after the spore suspension fully infiltrates into the solid medium; and S500: determining the number of viable spores according to the number of the observed fluorescent spots on the solid medium. The method is based on an α-glucosidase test method and can rapidly determine activity of the spore and judge activity of a single spore.

公开(公告)号：US20240385336A1

公开(公告)日：2024-11-21

申请号：US18689863

申请日：2023-05-06

Applicant: Zhejiang Cancer Hospital

Inventor： Wenjie WU ,  Guoping SHAN ,  Jinhui CHANG ,  Jiping LIU ,  Binbing WANG ,  Feng LU ,  Yixuan JIN ,  Xue BAI ,  Xiaolong CHENG ,  Xiaotong WANG

IPC: G01T1/20 ,  C12Q1/02 ,  G01T7/00

Abstract: The present disclosure discloses a method for measuring two-dimensional distribution of an industrial irradiation dose based on sprouting lag time of spores, and provides a film for measuring distribution of an industrial irradiation dose. The method includes the following steps: (1) manufacturing the film: spreading a layer of spore suspension flat on a plane of a carrier, and evaporating a suspension solvent to fix a layer of spore pixel surface on the plane of the carrier; covering the carrier with a glass lid to form a sprouting cavity to cover the spore pixel surface within the sprouting cavity; and providing the glass lid with a filling hole configured to add a sprouting agent; (2) obtaining a calibration curve; and (3) measuring the irradiation dose.

公开(公告)号：US11866707B2

公开(公告)日：2024-01-09

申请号：US18048451

申请日：2022-10-21

Applicant: ZHEJIANG CANCER HOSPITAL

Inventor： Lei Lei ,  Xiaojia Wang

IPC: C12N15/113 ,  A61P43/00

CPC classification number: C12N15/1135 ,  A61P43/00 ,  C12N2310/122

Abstract: This invention discloses the use of non-coding RNA SNHG17 as a biomarker and a therapeutic target that relates to the technical field of tumor biotherapy. This invention discloses a use of the non-coding RNA SNHG17 for the manufacture of a detection agent for predicting the drug resistance of fulvestrant. This invention also discloses the use of shRNA for inhibiting non-coding RNA SNHG17 expression. This shRNA can knockdown the expression of the non-coding RNA SNHG17 efficiently. In this way, the sensitivity of fulvestrant-resistant breast cancer cells can be increased. So this invention has good prospects for drug development.

公开(公告)号：US12131476B2

公开(公告)日：2024-10-29

申请号：US17571562

申请日：2022-01-10

Applicant: ZHEJIANG CANCER HOSPITAL

Inventor： Jie Zhang ,  Xue Bai ,  Guoping Shan

IPC: G06T7/00 ,  A61B5/06 ,  G06N3/04 ,  G06N3/045 ,  G06T7/246 ,  G06V10/75 ,  G16H40/60

CPC classification number: G06T7/0016 ,  A61B5/066 ,  G06N3/045 ,  G06T7/248 ,  G06V10/754 ,  G16H40/60 ,  G06T2207/10136 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30004

Abstract: Provided is a system and method for estimating the motion of a target inside a tissue based on surface deformation of the soft tissue. The system consists of an acquisition unit, a reference input unit, two surface extraction units, a target position extraction unit, a feature calculation unit, and a target motion estimation unit. The method includes: the acquisition unit acquires an image Ii of the soft tissue; the surface extraction unit extracts a surface fi of the soft tissue from Ii; the reference input unit acquires a reference image Iref of the soft tissue; the surface extraction unit and the target position extraction unit respectively extract a reference surface fref of the soft tissue and a target reference position tref from Iref, the feature calculation unit calculates deformation feature Ψi of fi relative to fref, the target motion estimation unit estimates the target displacement based on Ψi and tref.

公开(公告)号：US20230287504A1

公开(公告)日：2023-09-14

申请号：US17293382

申请日：2019-11-11

Applicant: Mirxes (Hangzhou) Biotechnology Co., Ltd. ,  Zhejiang Cancer Hospital

Inventor： Ruiyang Zou ,  Dan Su ,  He Cheng ,  Lisha Ying ,  Lihan Zhou

IPC: C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q2600/118

Abstract: Provided are peripheral blood miRNA markers for diagnosis of non-small cell lung cancer, wherein the peripheral blood miRNA markers comprise hsa-miR-1291, hsa-miR-1-3p, hsa-miR-214-3p, hsa-miR-375 or hsa-let-7a-5p. Five specific diagnostic markers suitable for diagnosing non-small cell lung cancer in Asian and Caucasian populations are validated on a large number of samples, and have higher population specificity compared to other miRNA markers previously reported. These five miRNA diagnostic markers are proposed for the first time and are shown to be more reliable than other miRNA molecular markers.

公开(公告)号：US20230374516A1

公开(公告)日：2023-11-23

申请号：US18048451

申请日：2022-10-21

Applicant: ZHEJIANG CANCER HOSPITAL

Inventor： Lei LEI ,  Xiaojia WANG

IPC: C12N15/113 ,  A61P43/00

CPC classification number: C12N15/1135 ,  A61P43/00 ,  C12N2310/122

Abstract: This invention discloses the use of non-coding RNA SNHG17 as a biomarker and a therapeutic target that relates to the technical field of tumor biotherapy. This invention discloses a use of the non-coding RNA SNHG17 for the manufacture of a detection agent for predicting the drug resistance of fulvestrant. This invention also discloses the use of shRNA for inhibiting non-coding RNA SNHG17 expression. This shRNA can knockdown the expression of the non-coding RNA SNHG17 efficiently. In this way, the sensitivity of fulvestrant-resistant breast cancer cells can be increased. So this invention has good prospects for drug development.

公开(公告)号：US20220130048A1

公开(公告)日：2022-04-28

申请号：US17571562

申请日：2022-01-10

Applicant: ZHEJIANG CANCER HOSPITAL

Inventor： Jie ZHANG ,  Xue BAI ,  Guoping SHAN

IPC: G06T7/00 ,  G06T7/246 ,  A61B5/06 ,  G16H40/60 ,  G06N3/04 ,  G06V10/75

Abstract: Provided is a system and method for estimating the motion of a target inside a tissue based on surface deformation of the soft tissue. The system consists of an acquisition unit, a reference input unit, two surface extraction units, a target position extraction unit, a feature calculation unit, and a target motion estimation unit. The method includes: the acquisition unit acquires an image Ii of the soft tissue; the surface extraction unit extracts a surface fi of the soft tissue from Ii; the reference input unit acquires a reference image Iref of the soft tissue; the surface extraction unit and the target position extraction unit respectively extract a reference surface fref of the soft tissue and a target reference position tref from Iref, the feature calculation unit calculates deformation feature Ψi of fi relative to fref, the target motion estimation unit estimates the target displacement based on Ψi and tref.