公开(公告)号：US20060094084A1

公开(公告)日：2006-05-04

申请号：US10531971

申请日：2003-12-10

申请人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

发明人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

IPC分类号： C12P19/28 ,  C12N1/21

CPC分类号： C12P17/182 ,  C12R1/15

摘要： The invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine (NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5′-xanthylic acid in culture medium at a high yield and concentration rate.

摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXSP 0201 KCCM 10448。 更具体地，本发明涉及具有抗渗透压的耐氨基酸棒杆菌KCCM 10340的突变菌株的产氨棒杆菌CJXSP 0201 KCCM 10448。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXSP 0201 KCCM 10448可以在早期培养中快速克服生长稳定期，并且在相同的发酵期内，可以以高产率和浓度的速率在培养基中积累5'-叶酸。

公开(公告)号：US07217558B2

公开(公告)日：2007-05-15

申请号：US10531675

申请日：2003-12-10

申请人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

发明人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

IPC分类号： C12N1/12 ,  C12N17/02 ,  C12N19/28 ,  C12N1/00

CPC分类号： C12P19/32 ,  C12P19/40 ,  C12R1/15

摘要： The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5′xanthylic acid in culture medium at a high yield and concentration rate.

摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXOL 0201 KCCM 10447。 更具体地，本发明涉及产氨杆菌CJXOL 0201 KCCM 10447，它是具有对寡霉素抗性的产氨棒杆菌KCCM10340的突变菌株。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXOL 0201 KCCM 10447可以在相同的发酵期内增加ATP的再生活性，并且可以以高产率和浓度的速率在培养基中积累5'-戊酸。

公开(公告)号：US06821768B2

公开(公告)日：2004-11-23

申请号：US10322455

申请日：2002-12-19

申请人： Jeong-Hwan Kim ,  Young-Hyeon Kwag ,  Jang-Hee Park ,  Eun-Sung Koh ,  Yoon-Suk Oh ,  Jea-Young Chang ,  Kwang-Ho Lee ,  Jae-Ick Sim ,  Jong-Kwon Han ,  Young-Hoon Park

发明人： Jeong-Hwan Kim ,  Young-Hyeon Kwag ,  Jang-Hee Park ,  Eun-Sung Koh ,  Yoon-Suk Oh ,  Jea-Young Chang ,  Kwang-Ho Lee ,  Jae-Ick Sim ,  Jong-Kwon Han ,  Young-Hoon Park

IPC分类号： C12N100

CPC分类号： C12R1/15 ,  C12N1/20 ,  C12P19/32 ,  Y10S435/843

摘要： The invention relates to microorganism producing 5′-xanthylic acid. A 3,4-dehydro-DL-proline resistant Corynebacterium ammoniagenes CJXPK001 strain is provided for producing 5′-xanthylic acid. It is a mutant strain of Corynebacterium ammoniagenes KFCC 10743 having a lower resistance to 3,4-dehydro-DL-proline. The mutant strain is not affected by osmotic pressure caused by accumulated, highly concentrated solute in a culture medium, and it can produce 5′-xanthylic acid at a high yield and concentration rate.

摘要翻译： 本发明涉及产生5'-戊酸的微生物。提供3,4-脱氢-DL-脯氨酸抗性产氨杆菌CJXPK001菌株以产生5'-戊酸。 它是具有对3,4-脱氢-DL-脯氨酸的较低抗性的产氨棒杆菌KFCC 10743的突变菌株。 突变株不受培养基中积累，高度浓缩的溶质引起的渗透压的影响，可以高产率和浓度产生5'-叶酸。

公开(公告)号：US07456010B2

公开(公告)日：2008-11-25

申请号：US10531971

申请日：2003-12-10

申请人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

发明人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

IPC分类号： C12N1/20

CPC分类号： C12P17/182 ,  C12R1/15

摘要： The invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine (NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5′-xanthylic acid in culture medium at a high yield and concentration rate.

摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXSP 0201 KCCM 10448。 更具体地，本发明涉及具有抗渗透压的耐氨基酸棒杆菌KCCM 10340的突变菌株的产氨棒杆菌CJXSP 0201 KCCM 10448。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXSP 0201 KCCM 10448可以在早期培养中快速克服生长稳定期，并且在相同的发酵期内，可以以高产率和浓度的速率在培养基中积累5'-叶酸。

公开(公告)号：US20060154346A1

公开(公告)日：2006-07-13

申请号：US10531675

申请日：2003-12-10

申请人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

发明人： Young-Hyeon Kwag ,  Ki-Hoon Oh ,  Jeong-Hwan Kim ,  Yoon-Suk Oh ,  Jae-Ick Sim ,  Young-Hoon Park ,  Jea-Young Chang

IPC分类号： C12P17/02 ,  C12N1/20

CPC分类号： C12P19/32 ,  C12P19/40 ,  C12R1/15

摘要： The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5′xanthylic acid in culture medium at a high yield and concentration rate.

摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXOL 0201 KCCM 10447。 更具体地，本发明涉及产氨杆菌CJXOL 0201 KCCM 10447，它是具有对寡霉素抗性的产氨棒杆菌KCCM10340的突变菌株。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXOL 0201 KCCM 10447可以在相同的发酵期内增加ATP的再生活性，并且可以高产率和浓度的速率在培养基中积累5'-戊酸。