公开(公告)号：US20120157824A1

公开(公告)日：2012-06-21

申请号：US13393472

申请日：2010-08-31

申请人： Stefan H. Bossmann ,  Deryl Troyer ,  Matthew T. Basel ,  Thilani Nishanthika Samarakoon ,  Hongwang Wang ,  Viktor Chikan ,  Franklin Orban Kroh ,  Olga Barbara Koper ,  Brandon Ray Walker ,  Xiaoxuan Leaym

发明人： Stefan H. Bossmann ,  Deryl Troyer ,  Matthew T. Basel ,  Thilani Nishanthika Samarakoon ,  Hongwang Wang ,  Viktor Chikan ,  Franklin Orban Kroh ,  Olga Barbara Koper ,  Brandon Ray Walker ,  Xiaoxuan Leaym

IPC分类号： A61K49/18 ,  A61B5/055

CPC分类号： C12Q1/37 ,  G01N33/54346

摘要： The present invention provides multifunctional nanoplatforms for assessing the activity of a protease in vivo or in vitro, along with methods of imaging and detecting the presence of cancerous or precancerous tissues, and the therapeutic treatment thereof, including monitoring of treatment. The diagnostic nanoplatforms comprise nanoparticles and are linked to each other or other particles via an oligopeptide linkage that comprises a consensus sequence specific for the target protease. Cleavage of the sequence by the target protease can be detected using various sensors, and the diagnostic results can be correlated with cancer prognosis. Individual unlinked nanoplatforms are also adaptable for therapeutic hyperthermia treatment of the cancerous tissue.

摘要翻译： 本发明提供用于评估体内或体外蛋白酶活性的多功能纳米平板，以及成像和检测癌或癌前组织的存在及其治疗方法，包括治疗的监测。 诊断性纳米平台包含纳米颗粒，并通过包含靶蛋白酶特异性共有序列的寡肽连接物彼此连接或其它颗粒。 可以使用各种传感器检测目标蛋白酶的序列的切割，并且诊断结果可以与癌症预后相关。 单独的未连接的纳米平台也适用于癌组织的治疗性高热治疗。