公开(公告)号：US07592179B2

公开(公告)日：2009-09-22

申请号：US11872481

申请日：2007-10-15

申请人： Xiaonan Guo

发明人： Xiaonan Guo

IPC分类号： G01N33/48 ,  G01N33/96

CPC分类号： G01N33/56972 ,  G01N33/96 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525

摘要： The present invention provides a method for preparing a five-part differential white blood cell control and a white blood cell control prepared thereby. The white blood cell control obtained by the method of the present invention perfectly retains the light scattering properties of every type of white blood cells and has much higher stability than that of real white blood cells, and therefore can be used for the quality control of five-part differential hematology analyzers based on the principle of multi-angle light scattering.

摘要翻译： 本发明提供一种制备由其制备的五部分差异白细胞对照和白细胞对照的方法。 通过本发明的方法获得的白细胞控制完全保留了每种白细胞的光散射特性，并且具有比真实白细胞更稳定的稳定性，因此可用于五 - 基于多角度光散射原理的差示血液分析仪。

公开(公告)号：US07659122B2

公开(公告)日：2010-02-09

申请号：US11872522

申请日：2007-10-15

申请人： Xiaonan Guo

发明人： Xiaonan Guo

IPC分类号： G01N33/48 ,  G01N33/96

CPC分类号： G01N33/96 ,  G01N33/56972 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： Disclosed is a single population of simulated leukocyte granules prepared from mammalian whole white blood cells, which is useful in calibrators for a hematology analyzer, a calibrator which includes the single population of simulated leukocyte granules. Disclosed is also a method of preparing the single population of simulated leukocyte granules. The method of preparing single population of simulated leukocyte granules includes the actions of: obtaining mammalian whole white blood cells; treating the mammalian whole white blood cells with a hemolytic agent and a leukocyte treating liquid which includes an isotonic saline solution. The isotonic saline solution may include a fixative, an oxidizer, and a carbohydrate compound; fixing the treated mammalian whole white blood cells with a fixative; and suspending the fixed cells in a preservation medium for long-term preservation of fixed cells.

摘要翻译： 公开了由哺乳动物全白细胞制备的单一模拟白细胞颗粒群，其可用于血液分析仪的校准器，包括单个模拟白细胞颗粒群的校准器。 还公开了制备单一模拟白细胞颗粒群体的方法。 制备模拟白细胞颗粒的单一群体的方法包括：获得哺乳动物全血白细胞的作用; 用包含等渗盐水溶液的溶血剂和白细胞处理液处理哺乳动物全血白细胞。 等渗盐水溶液可包括固定剂，氧化剂和碳水化合物; 用固定剂固定经处理的哺乳动物全白血细胞; 并将固定的细胞悬浮在保存介质中以长期保存固定的细胞。

公开(公告)号：US20080293144A1

公开(公告)日：2008-11-27

申请号：US11872481

申请日：2007-10-15

申请人： Xiaonan Guo

发明人： Xiaonan Guo

IPC分类号： G01N33/96 ,  G01N33/48

CPC分类号： G01N33/56972 ,  G01N33/96 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525

摘要： The present invention provides a method for preparing a five-part differential white blood cell control and a white blood cell control prepared thereby. The white blood cell control obtained by the method of the present invention perfectly retains the light scattering properties of every type of white blood cells and has much higher stability than that of real white blood cells, and therefore can be used for the quality control of five-part differential hematology analyzers based on the principle of multi-angle light scattering.

摘要翻译： 本发明提供一种制备由其制备的五部分差异白细胞对照和白细胞对照的方法。 通过本发明的方法获得的白细胞控制完全保留了每种白细胞的光散射特性，并且具有比真实白细胞更稳定的稳定性，因此可用于五 - 基于多角度光散射原理的差示血液分析仪。

公开(公告)号：US20080293145A1

公开(公告)日：2008-11-27

申请号：US11872522

申请日：2007-10-15

申请人： Xiaonan Guo

发明人： Xiaonan Guo

IPC分类号： G01N37/00 ,  G01N33/49

CPC分类号： G01N33/96 ,  G01N33/56972 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention discloses a single population of simulated leukocyte granules prepared from mammalian whole white blood cells, which is useful in calibrators for a hematology analyzer, a calibrator comprising the single population of simulated leukocyte granules, and a method of preparing the single population of simulated leukocyte granules. The method of preparing single population of simulated leukocyte granules according to present invention comprises the steps of: obtaining mammalian whole white blood cells; treating the mammalian whole white blood cells under the action of a hemolytic agent in the presence of a leukocyte treating liquid, i.e., an isotonic saline solution comprising a fixative, a oxidizer and a carbohydrate; fixing the treated mammalian whole white blood cells with a fixative; and suspending the fixed cells in a preservation medium suitable for long-term preservation of fixed cells.

摘要翻译： 本发明公开了由哺乳动物全白血细胞制备的单个模拟白细胞颗粒群，其可用于血液分析仪的校准器，包含单粒模拟白细胞颗粒的校准物，以及制备模拟白细胞单个群体的方法 白细胞颗粒。 根据本发明制备单粒模拟白细胞颗粒的方法包括以下步骤：获得哺乳动物全血白细胞; 在白细胞处理液体即包含固定剂，氧化剂和碳水化合物的等渗盐水溶液的存在下，在溶血剂的作用下处理哺乳动物全血白细胞; 用固定剂固定经处理的哺乳动物全白血细胞; 并将固定的细胞悬浮在适于长期保存固定细胞的保存介质中。