公开(公告)号：US20040214331A1

公开(公告)日：2004-10-28

申请号：US10732345

申请日：2003-12-11

申请人： The Universtiy of Queensland and CSL Limited

发明人： Ian Frazer ,  Jian Zhou

IPC分类号： C12N015/86 ,  C12N007/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2710/20043 ,  C12Q1/701 ,  Y10S977/802

摘要： A method of providing papillomavirus like particles which may be used for diagnostic purposes or for incorporation in a vaccine for use in relation to infections causd by papillomavirus. The method includes an initial step of constructing one or more recombinant DNA molecules which each encode papillomavirus L1 protein or a combination of papillomavirus L1 protein and papillomavirus L2 protein followed by a further step of transfecting a suitable host cell with one or more of the recombinant DNA molecules so that virus like particles (VLPs) are produced within the cell after expression of the L1 or combination of L1 and L2 proteins. The VLPs are also claimed per se as well as vaccines incorporating the VLPs.

摘要翻译： 提供乳头瘤病毒样颗粒的方法，其可用于诊断目的或用于结合用于与由乳头瘤病毒引起的感染有关的疫苗中。 该方法包括构建一个或多个重组DNA分子的初始步骤，每个重组DNA分子编码乳头瘤病毒L1蛋白或乳头瘤病毒L1蛋白和乳头瘤病毒L2蛋白的组合，接着进一步用一种或多种重组DNA转染合适的宿主细胞 分子，使得在表达L1或L1和L2蛋白的组合之后，在细胞内产生病毒样颗粒（VLP）。 本身还要求VLP以及结合VLP的疫苗。