公开(公告)号：US20120244602A1

公开(公告)日：2012-09-27

申请号：US13485085

申请日：2012-05-31

申请人： Taisei OKUMURA ,  Mamoru NUMATA ,  Shuzo KOJIMA ,  Yasuhisa NAGATA ,  Naoki TAHARA ,  Hiroyuki KUROKI ,  Masato KUKIZAKI ,  Tomohiro TANAKA

发明人： Taisei OKUMURA ,  Mamoru NUMATA ,  Shuzo KOJIMA ,  Yasuhisa NAGATA ,  Naoki TAHARA ,  Hiroyuki KUROKI ,  Masato KUKIZAKI ,  Tomohiro TANAKA

IPC分类号： C12N1/20 ,  C12M1/04 ,  C12N1/12 ,  C12N1/16 ,  C12N1/14

CPC分类号： C12N1/20 ,  B01F3/0412 ,  B01F3/04262 ,  B01F7/00383 ,  B01F7/1625

摘要： A method for culturing cells or microorganisms by dissolving oxygen or carbon dioxide in a culture solution containing a nutrient including: supplying gas containing oxygen or carbon dioxide into inside of a glass porous body being configured to have cylindrical shape and a large number of uniformly fine pores in the outer surface and being configured to seal the end of the glass porous body; generating bubbles that have a 50% diameter of 200 μm or less in a volume-based particle size distribution from the outer surface of the porous body by using gas containing oxygen or carbon dioxide that is supplied from an unsealed end of the glass porous body; suppressing aggregation of the bubbles by at least one of a cell-protecting agent for protecting the cells and a protein hydrolysate included in the culture solution; and dissolving oxygen or carbon dioxide in the culture solution.

摘要翻译： 一种通过将氧或二氧化碳溶解在含有营养物的培养液中培养细胞或微生物的方法，包括：将含有氧或二氧化碳的气体供给到玻璃多孔体的内部，该玻璃多孔体被构造成具有圆筒形状和大量均匀的细孔 在外表面上并被构造成密封玻璃多孔体的端部; 通过使用从玻璃多孔体的未密封端供给的含有氧气或二氧化碳的气体，从多孔体的外表面产生体积型粒径分布中的直径为200μm以下的直径为200μm以下的气泡; 通过保护细胞的细胞保护剂和培养液中包含的蛋白质水解物中的至少一种来抑制气泡的聚集; 并将氧或二氧化碳溶解在培养液中。