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公开(公告)号：US20200206337A1
公开(公告)日：2020-07-02
申请号：US16684086
申请日：2019-11-14
发明人： Germán Bou Arévalo , María Clara Póvoa Cabral , Astrid Pérez Gómez , María Merino Carballeira , Alejandro Beceiro Casas , Patricia García Fernández
IPC分类号： A61K39/104 , A61K39/085 , C12N1/36 , C12N9/10 , C12N9/90 , C07K16/12 , G01N33/569 , A61P31/04 , C12R1/385 , C12R1/01 , C12R1/445
摘要： The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wall, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
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公开(公告)号：US10517939B2
公开(公告)日：2019-12-31
申请号：US15028176
申请日：2014-10-13
发明人： Germán Bou Arévalo , María Clara Póvoa Cabral , Astrid Pérez Gómez , María Merino Carballeira , Alejandro Beceiro Casas , Patricia García Fernández
IPC分类号： A61K39/104 , A61K39/085 , C12R1/385 , C12N1/36 , C07K16/12 , A61K39/00 , C12R1/01 , C12R1/445 , C12N9/10 , C12N9/90 , G01N33/569
摘要： The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
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公开(公告)号：US11197922B2
公开(公告)日：2021-12-14
申请号：US16684086
申请日：2019-11-14
发明人： Germán Bou Arévalo , María Clara Póvoa Cabral , Astrid Pérez Gómez , María Merino Carballeira , Alejandro Beceiro Casas , Patricia García Fernández
IPC分类号： A61K39/085 , A61K39/00 , C07K16/12 , A61K39/104 , C12N1/36 , C12N9/10 , C12N9/90 , G01N33/569 , A61P31/04 , C12R1/01 , C12R1/385 , C12R1/445
摘要： The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wall, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
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公开(公告)号：US20160235834A1
公开(公告)日：2016-08-18
申请号：US15028176
申请日：2014-10-13
发明人： Germán Bou Arévalo , María Clara Póvoa Cabral , Astrid Pèrez Gómez , María Merino Carballeira , Alejandro Beceiro Casas , Patricia García Fernández
IPC分类号： A61K39/104 , C07K16/12 , G01N33/569 , C12N9/10 , C12N9/90 , A61K39/085 , C12N1/36
摘要： The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
摘要翻译： 本发明涉及一种生产适合作为疫苗候选物的活减毒细菌菌株的方法，其包括以下步骤：A.提供能够表达谷氨酸消旋酶和可能的D-氨基酸转氨酶并包含肽聚糖细胞的细菌菌株 ，以及B.使编码谷氨酸消旋酶的基因或基因失活，如果需要，编码D-氨基酸转氨酶的基因或基因使得细菌菌株不再能够表达功能性谷氨酸消旋酶 和/或功能性D-氨基酸转氨酶; 其中所述基因的失活导致所述细菌菌株对于D-谷氨酸是营养缺陷型的。
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