公开(公告)号：US20060269974A1

公开(公告)日：2006-11-30

申请号：US11411246

申请日：2006-04-26

申请人： Raymond Dwek ,  Umi Hamid ,  Rafael Llorens ,  Rosa Peracaula ,  Catherine Radcliffe ,  John Robertson ,  Louise Royle ,  Pauline Rudd ,  Nicole Zitzmann

发明人： Raymond Dwek ,  Umi Hamid ,  Rafael Llorens ,  Rosa Peracaula ,  Catherine Radcliffe ,  John Robertson ,  Louise Royle ,  Pauline Rudd ,  Nicole Zitzmann

IPC分类号： G01N33/574 ,  C12Q1/37 ,  G01N33/00

CPC分类号： G01N33/6842 ,  G01N33/57407 ,  G01N33/57484

摘要： One can identify and quantify one or more glycosylation markers of cancer by utilizing quantitative HPLC analysis of glycans which have been released from unpurified glycoproteins. The unpurified glycoproteins can be total glycoproteins or a selection of the total glycoproteins. The identified glycosylation marker can be a native glycan or a digestion product which has been segregated and amplified by exoglycosidase digestions. One can utilize the identified glycosylation marker, for example, for diagnosing and/or monitoring cancer in a subject. One can also use the glycosylation marker to identify glycoprotein biomarkers that carry the glycosylation marker. Such biomarkers can also be used for monitoring and/or diagnosing cancer. The biomarker may also be a subset of glycoforms of a glycoprotein that are separated in trains of spots on 2D gel.

摘要翻译： 通过利用已经从未纯化的糖蛋白释放的聚糖的定量HPLC分析，可以鉴定和定量癌症的一种或多种糖基化标记物。 未纯化的糖蛋白可以是总糖蛋白或总糖蛋白的选择。 鉴定的糖基化标记可以是天然聚糖或消化产物，其通过去糖基酶消化分离和扩增。 可以使用鉴定的糖基化标记，例如用于诊断和/或监测受试者的癌症。 也可以使用糖基化标记来鉴定携带糖基化标记的糖蛋白生物标志物。 此类生物标志物也可用于监测和/或诊断癌症。 生物标志物也可以是在2D凝胶上的斑点列中分离的糖蛋白的糖形成的子集。