公开(公告)号：US06753155B1

公开(公告)日：2004-06-22

申请号：US09482604

申请日：2000-01-14

申请人： Prabhati Ray

发明人： Prabhati Ray

IPC分类号： G01N33573

CPC分类号： G01N33/573 ,  G01N2333/95

摘要： This invention relates to the discovery that toxicity to mustard may be evaluated by diagnostic test means disclosed herein. Upon electrophoretic separation (sodium dodocyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)) of buffered extract of human skin cells (normal human epidermal keratinocytes (NHEK)) which had been exposed to mustard-type chemical compounds a band at approximately 50,000 to 80,000 daltons molecular weight was found. The protein band constitutes a biomarker.

摘要翻译： 本发明涉及通过本文公开的诊断测试手段评估对芥子的毒性的发现。 在已经暴露于芥菜型化合物的人皮肤细胞缓冲提取物（正常人表皮角质形成细胞（NHEK））上进行电泳分离（十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE））约50,000至80,000道尔顿 发现分子量。 蛋白质带构成生物标志物。

公开(公告)号：US6124108A

公开(公告)日：2000-09-26

申请号：US855268

申请日：1997-05-13

申请人： Prabhati Ray

发明人： Prabhati Ray

IPC分类号： G01N33/573

CPC分类号： G01N33/573 ,  G01N2333/95

摘要： This invention relates to the discovery that toxicity to mustard may be euated by diagnostic test means disclosed herein. Upon electrophoretic separation (sodium dodocyl sulfate polyacryl-amide gel electrophoresis (SDS-PAGE)) of buffered extract of human skin cells (normal human epidermal keratinocytes (NHEK)) which had been exposed to mustard-type chemical compounds a band at approximately 50,000 to 80,000 daltons molecular weight was found. The protein band constitutes a biomarker. The marker protein can be used either to raise protective antibodies to protect against the protease or may be used in a kit for identifying presence or absence of the marker in study of tissues taken from individuals who may have been exposed to mustard poisoning.

摘要翻译： 本发明涉及通过本文公开的诊断测试手段评估对芥子的毒性的发现。 在已经暴露于芥菜型化合物的人类皮肤细胞（正常人​​表皮角质形成细胞（NHEK））的缓冲提取物的电泳分离（十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE））上， 发现80,000道尔顿分子量。 蛋白质带构成生物标志物。 标记蛋白可用于提高保护性抗体以防止蛋白酶，或者可用于鉴定存在或不存在可能已经暴露于芥菜中毒的个体的组织的研究中存在或不存在标记物的试剂盒中。