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    METHOD TO GROW LAWSONIA INTRACELLULARIS BACTERIA IN PERSISTENTLY INFECTED MCCOY CELLS
    3.
    发明申请
    METHOD TO GROW LAWSONIA INTRACELLULARIS BACTERIA IN PERSISTENTLY INFECTED MCCOY CELLS 有权
    在不断感染的MCCOY细胞中培养LAWSONIA细胞内细菌的方法

    公开(公告)号：US20120213739A1

    公开(公告)日：2012-08-23

    申请号：US13505667

    申请日：2010-11-08

    申请人： Yvonne Maria Johanna Corina Biermann ,  Carla Christina Schrier ,  Petrus Theodorus Johannes Andries van Gelder ,  Bram Verdellen ,  Barry Lijsdonk

    发明人： Yvonne Maria Johanna Corina Biermann ,  Carla Christina Schrier ,  Petrus Theodorus Johannes Andries van Gelder ,  Bram Verdellen ,  Barry Lijsdonk

    IPC分类号： A61K35/74 ,  C12N1/20 ,  A61P31/04 ,  C12N5/071

    CPC分类号： C12N1/20 ,  C12N1/00

    摘要： The present invention pertains to a method to obtain McCoy cells persistently infected with Lawsonia intracellularis bacteria, comprising infecting McCoy cells with Lawsonia intracellularis bacteria, growing the infected McCoy cells in a suitable medium at an oxygen concentration less than 18% to arrive at a culture of McCoy cells infected with Lawsonia intracellularis bacteria, passing at least a part of the said culture to fresh medium, and without adding uninfected McCoy cells to the medium, growing the infected McCoy cells contained in the said at least part in the fresh medium at an oxygen concentration less than 18%, to obtain the persistently infected McCoy cells. The invention also pertains to the use of such persistently infected cells to grow and obtain these bacteria in purified form.

    摘要翻译： 本发明涉及一种获得持续感染细胞内劳森氏菌细菌的McCoy细胞的方法，包括用细胞内劳森氏菌感染McCoy细胞，将感染的McCoy细胞在氧浓度小于18％的合适培养基中培养以获得 McCoy细胞感染细胞内劳森氏菌细菌，将至少一部分所述培养物转移到新鲜培养基中，并且不将未感染的McCoy细胞添加到培养基中，在含氧的新鲜培养基中培养所述至少部分所述至少部分的感染的McCoy细胞 浓度小于18％，以获得持续感染的McCoy细胞。 本发明还涉及使用这种持续感染的细胞生长并获得纯化形式的这些细菌。