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    HYPERURICEMIA MODEL
    2.
    发明申请
    HYPERURICEMIA MODEL 审中-公开
    HYPERURICEMIA模型

    公开(公告)号：US20170055504A1

    公开(公告)日：2017-03-02

    申请号：US15308116

    申请日：2015-05-07

    申请人： PHOENIXBIO CO., LTD.

    发明人： Masakazu KAKUNI ,  Yumiko IWASAKI ,  Chise MUKAIDANI

    IPC分类号： A01K67/027 ,  G01N33/50

    CPC分类号： A01K67/0271 ,  A01K67/0276 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0362 ,  G01N33/50 ,  G01N33/5088 ,  G01N2500/00 ,  G01N2800/708

    摘要： Provided is a non-human animal that is highly practical as a hyperuricenia model, the non-human animal being the following: (a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or (b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.

    摘要翻译： 提供作为高尿酸血症模型非常实用的非人类动物，非人类动物如下：（a）通过将人肝细胞移植到一个或多个第一嵌合非人动物中而获得的非人动物 具有肝功能障碍的免疫缺陷型非人动物; 并且随后将所述含嘌呤碱的物质施用于所述一级嵌合非人动物，或（b）通过两步生产连续移植的嵌合非人动物得到的非人动物，第一步是产生 通过将肝细胞移植到具有肝功能障碍的免疫缺陷型非人动物中的第一嵌合非人动物，第二步是将生长在初级嵌合非人动物体内的人肝细胞移植到免疫缺陷型非人动物的步骤， - 具有肝功能障碍的人类动物，第二步执行一次或多次; 并随后向连续移植的嵌合非人动物施用含嘌呤碱的物质。

    POLYNUCLEOTIDE DERIVED FROM NOVEL HEPATITIS C VIRUS STRAIN AND USE THEREOF
    3.
    发明申请
    POLYNUCLEOTIDE DERIVED FROM NOVEL HEPATITIS C VIRUS STRAIN AND USE THEREOF 审中-公开
    从新型丙型肝炎病毒株衍生的多核苷酸及其用途

    公开(公告)号：US20140178856A1

    公开(公告)日：2014-06-26

    申请号：US14078903

    申请日：2013-11-13

    申请人： PHOENIXBIO CO., LTD. ,  TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE

    发明人： Michinori KOHARA ,  Masaaki ARAI ,  Chise MUKAIDANI

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/707 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12N2770/24251 ,  G01N2333/18 ,  G01N2500/10

    摘要： A polynucleotide encoding the amino acid shown in SEQ ID NO:2 or SEQ ID NO: 5, or encoding an amino acid sequence having not less than 98% identity thereto; preferably a polynucleotide comprising replacement of the amino acid corresponding to glutamic acid at position 1202 of SEQ ID NO:2 (position 177 of SEQ ID NO:5) with glycine, replacement of the amino acid corresponding to glutamic acid at position 1056 (position 31 of SEQ ID NO:5) with valine, and replacement of the amino acid corresponding to alanine at position 2199 (position 1174 of SEQ ID NO:5) with threonine.

    摘要翻译： 编码SEQ ID NO：2或SEQ ID NO：5所示氨基酸的多核苷酸，或编码与其不同于98％同一性的氨基酸序列; 优选包括将SEQ ID NO：2（SEQ ID NO：5的第177位）的1202位相应于谷氨酸的氨基酸与甘氨酸取代的多核苷酸，替换对应于1056位谷氨酸的氨基酸（位置31 SEQ ID NO：5）与缬氨酸的氨基酸取代，并且用苏氨酸置换对应于位置2199（SEQ ID NO：5的位置1174）的丙氨酸。

    Mouse model of hyperuricemia
    6.
    发明授权
    Mouse model of hyperuricemia 有权

    公开(公告)号：US10314295B2

    公开(公告)日：2019-06-11

    申请号：US15308116

    申请日：2015-05-07

    申请人： PHOENIXBIO CO., LTD.

    发明人： Masakazu Kakuni ,  Yumiko Iwasaki ,  Chise Mukaidani

    IPC分类号： A01K67/027 ,  G01N33/50

    摘要： Provided is a non-human animal that is highly practical as a hyperuricenia model, the non-human animal being the following: (a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or (b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.

    UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE
    8.
    发明申请
    UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE 有权
    尿激酶型PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE

    公开(公告)号：US20150128298A1

    公开(公告)日：2015-05-07

    申请号：US14397091

    申请日：2013-04-25

    申请人： TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE ,  CHUGAI SEIYAKU KABUSHIKI KAISHA ,  PHOENIXBIO CO., LTD.

    发明人： Michinori Kohara ,  Koichi Jishage ,  Yosuke Kawase ,  Chise Mukaidani ,  Hiroki Oshita ,  Satoko Hamamura

    IPC分类号： A01K67/027 ,  C12N15/877 ,  C12N15/85 ,  G01N33/50

    CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/0337 ,  A01K2267/035 ,  C12N9/6462 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2015/8581 ,  G01N33/5067 ,  G01N33/5088 ,  Y10T436/146666

    摘要： The present invention provides a mouse with liver damage, having a high degree of damage against the mouse's original hepatocytes while having a uPA gene in a heterozygous form, and a method for efficiently preparing the mouse. Specifically, the method for preparing a mouse with liver damage having the uPA gene in a heterozygous form comprises the following steps of:(i) transforming mouse ES cells with a DNA fragment containing a liver-specific promoter/enhancer and cDNA that encodes a urokinase-type plasminogen activator operably linked under the control thereof; (ii) injecting the transformed mouse ES cells obtained in step (i) into a host embryo; (iii) transplanting the host embryo obtained in step (ii) via the injection of the ES cells into the uterus of a surrogate mother mouse, so as to obtain a chimeric mouse; and (iv) crossing the chimeric mice obtained in step (iii), so as to obtain a transgenic mouse in which the DNA fragment is introduced in a heterozygous form.

    摘要翻译： 本发明提供了具有肝损伤的小鼠，其具有对小鼠原始肝细胞的高度损伤，同时具有杂合形式的uPA基因，以及有效制备小鼠的方法。 具体而言，制备具有uPA基因的肝损伤的小鼠具有杂合形式的方法包括以下步骤：（i）用含有肝特异性启动子/增强子的DNA片段和编码尿激酶的cDNA转化小鼠ES细胞 型纤维蛋白溶酶原激活剂在其控制下可操作地连接; （ii）将步骤（i）中获得的转化的小鼠ES细胞注入宿主胚胎; （iii）通过将ES细胞注射到替代母亲小鼠的子宫中移植步骤（ii）中获得的宿主胚，以获得嵌合小鼠; 和（iv）与步骤（iii）中获得的嵌合小鼠杂交，以获得其中DNA片段以杂合形式引入的转基因小鼠。