公开(公告)号：US20130302887A1

公开(公告)日：2013-11-14

申请号：US13947884

申请日：2013-07-22

Applicant: Michael Shamblott ,  Michael Cohen

Inventor： Michael Shamblott ,  Michael Cohen

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2502/04 ,  C12N2533/54

Abstract: Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic gem (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived.

Abstract translation: 通过使用从人胚胎宝石（EG）细胞衍生物（例如胚状体衍生细胞）的培养基获得的分泌产物，提供用于衍生和培养人胚胎干（ES）细胞并维持其培养多能性的方法。 底物包括诸如胶原I，纤连蛋白或超纤连蛋白的化合物，或通常人衍生的细胞外基质。

公开(公告)号：US20130071926A1

公开(公告)日：2013-03-21

申请号：US13675708

申请日：2012-11-13

Applicant: Michael Shamblott ,  Michael Cohen

Inventor： Michael Shamblott ,  Michael Cohen

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2502/04 ,  C12N2533/54

Abstract: Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic germ (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived.

Abstract translation: 通过利用从人类胚胎（EG）细胞衍生物（例如胚状体衍生细胞）的培养基获得的分泌产物，提供用于诱导和培养人胚胎干（ES）细胞并保持其培养多能性的方法。 底物包括诸如胶原I，纤连蛋白或超纤连蛋白的化合物，或通常人衍生的细胞外基质。