公开(公告)号：US20100323428A1

公开(公告)日：2010-12-23

申请号：US12526211

申请日：2008-02-07

Applicant: Mariko Yoshizaki ,  Makota Inoue ,  Mamoru Hasegawa

Inventor： Mariko Yoshizaki ,  Makota Inoue ,  Mamoru Hasegawa

IPC: C12N7/01 ,  C12N15/63

CPC classification number: C12N7/00 ,  A61K48/00 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2760/18822 ,  C12N2760/18861

Abstract: An objective of the present invention is to provide attenuated minus-strand RNA viruses. The present inventors discovered that the amino acid mutation at position 1214 (Y1214F) in the amino acid sequence of L protein of Sendai virus suppressed the viral genome replication activity and/or transcription activity. The inventors also found that the deletion of a particular gene from the viral genome could result in much less cytotoxicity and immune response than conventional. The inventors thus completed the present invention.

Abstract translation: 本发明的目的是提供减毒的负链RNA病毒。 本发明人发现仙台病毒L蛋白的氨基酸序列中第1214位氨基酸突变（Y1214F）抑制病毒基因组复制活性和/或转录活性。 本发明人还发现，从病毒基因组中删除特定基因可导致远低于常规的细胞毒性和免疫应答。 本发明人完成了本发明。