公开(公告)号：US08846032B2

公开(公告)日：2014-09-30

申请号：US13595897

申请日：2012-08-27

申请人： Jonathan Liu ,  Richard Schwartz ,  Mark Thompson ,  Luis Maranga ,  Mridul Ghosh ,  Ajit Subramanian ,  Simon Sheng-Tsiung Hsu

发明人： Jonathan Liu ,  Richard Schwartz ,  Mark Thompson ,  Luis Maranga ,  Mridul Ghosh ,  Ajit Subramanian ,  Simon Sheng-Tsiung Hsu

IPC分类号： A61K39/145 ,  A01N65/00 ,  A61K39/00 ,  C12N5/02 ,  C07K17/00 ,  C12N7/00

CPC分类号： C12N7/00 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16251 ,  C12N2760/16252

摘要： The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.

摘要翻译： 本发明涉及可以在细胞培养中将病毒（例如流感病毒）生长至比先前可能更高滴度的新型MDCK细胞。 MDCK细胞可适应于无血清培养基。 本发明还涉及包含MDCK细胞的细胞培养组合物和用于培养MDCK细胞的培养方法。 本发明还涉及使用本发明的MDCK细胞在细胞培养中生产流感病毒的方法。