公开(公告)号：US08658401B2

公开(公告)日：2014-02-25

申请号：US13071126

申请日：2011-03-24

申请人： Yuanfa Liu ,  Kangyi Zhang ,  Xingguo Wang ,  Xiangyun Qian ,  Li Zhou ,  Zhihua Song

发明人： Yuanfa Liu ,  Kangyi Zhang ,  Xingguo Wang ,  Xiangyun Qian ,  Li Zhou ,  Zhihua Song

IPC分类号： C12P9/00

CPC分类号： C12P9/00 ,  C12P13/001

摘要： Disclosed is a method for preparing L-α-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A1-based enzymatic hydrolysis, ion-exchange resin purification and silica gel column chromatography to prepare L-α-glycerylphosphorylcholin with purity up to 99.8% and a final yield up to 78.4%. The method disclosed is simple, cost-effective, environmentally friendly, and adaptable to industrial applications.

摘要翻译： 公开了以高产率和纯度制备L-α-甘油基磷酰胆碱的方法。 该方法采用磷脂酶A1酶水解，离子交换树脂纯化和硅胶柱色谱法制备纯度高达99.8％的L-α-甘油基磷酰胆碱，最终产率高达78.4％。 所公开的方法简单，成本效益高，环保，适用于工业应用。

公开(公告)号：US20120244583A1

公开(公告)日：2012-09-27

申请号：US13071126

申请日：2011-03-24

申请人： Yuanfa Liu ,  Kangyi Zhang ,  Xingguo Wang ,  Xiangyun Qian ,  Li Zhou ,  Zhihua Song

发明人： Yuanfa Liu ,  Kangyi Zhang ,  Xingguo Wang ,  Xiangyun Qian ,  Li Zhou ,  Zhihua Song

IPC分类号： C12P13/00

CPC分类号： C12P9/00 ,  C12P13/001

摘要： Disclosed is a method for preparing L-α-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A1-based enzymatic hydrolysis, ion-exchange resin purification and silica gel column chromatography to prepare L-α-glycerylphosphorylcholin with purity up to 99.8% and a final yield up to 78.4%. The method disclosed is simple, cost-effective, environmentally friendly, and adaptable to industrial applications.

摘要翻译： 公开了以高产率和纯度制备L-α-甘油基磷酰胆碱的方法。 该方法采用磷脂酶A1酶水解，离子交换树脂纯化和硅胶柱色谱法制备纯度高达99.8％的L-α-甘油基磷酰胆碱，最终产率高达78.4％。 所公开的方法简单，成本效益高，环保，适用于工业应用。