公开(公告)号：US09260508B2

公开(公告)日：2016-02-16

申请号：US12643286

申请日：2009-12-21

申请人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

发明人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

IPC分类号： C40B30/04 ,  A61K39/00 ,  C07K16/00 ,  C07K16/28

CPC分类号： C07K16/28 ,  A61K2039/505 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/75 ,  C07K2317/92

摘要： The present invention relates to a method for generating immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored. The invention also provides immunoglobulin sequences obtainable by the method of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More specifically, the present invention relates to generation of immunoglobulin sequences in camelids, preferably directed against cell-associated antigens, in particular antigens with multiple transmembrane spanning domains, including GPCRs and ion channels, by DNA vaccination. Furthermore, the present invention relates to said immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored, such as e.g. GPCRs and ion channels, more preferably ion channels.

摘要翻译： 本发明涉及产生针对细胞相关抗原的免疫球蛋白序列的方法，更具体地说，涉及膜锚定的抗原。 本发明还提供可通过本发明的方法获得的免疫球蛋白序列。 具体地说，本发明涉及使用DNA疫苗接种产生免疫球蛋白序列。 更具体地，本发明涉及通过DNA疫苗接种在骆驼中产生免疫球蛋白序列，优选针对细胞相关抗原，特别是具有多个跨膜跨越结构域的抗原，包括GPCR和离子通道。 此外，本发明涉及针对细胞相关抗原的所述免疫球蛋白序列，更具体地，涉及膜锚定的抗原，例如抗体。 GPCR和离子通道，更优选离子通道。

公开(公告)号：US20100173799A1

公开(公告)日：2010-07-08

申请号：US12643286

申请日：2009-12-21

申请人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

发明人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

IPC分类号： C40B30/04

CPC分类号： C07K16/28 ,  A61K2039/505 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/75 ,  C07K2317/92

摘要： The present invention relates to a method for generating immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored. The invention also provides immunoglobulin sequences obtainable by the method of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More specifically, the present invention relates to generation of immunoglobulin sequences in camelids, preferably directed against cell-associated antigens, in particular antigens with multiple transmembrane spanning domains, including GPCRs and ion channels, by DNA vaccination. Furthermore, the present invention relates to said immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored, such as e.g. GPCRs and ion channels, more preferably ion channels.

摘要翻译： 本发明涉及产生针对细胞相关抗原的免疫球蛋白序列的方法，更具体地说，涉及膜锚定的抗原。 本发明还提供可通过本发明的方法获得的免疫球蛋白序列。 具体地说，本发明涉及使用DNA疫苗接种产生免疫球蛋白序列。 更具体地，本发明涉及通过DNA疫苗接种在骆驼中产生免疫球蛋白序列，优选针对细胞相关抗原，特别是具有多个跨膜跨越结构域的抗原，包括GPCR和离子通道。 此外，本发明涉及针对细胞相关抗原的所述免疫球蛋白序列，更具体地，涉及膜锚定的抗原，例如抗体。 GPCR和离子通道，更优选离子通道。