公开(公告)号：US20110078823A1

公开(公告)日：2011-03-31

申请号：US12888636

申请日：2010-09-23

申请人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

发明人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

公开(公告)号：US06784340B1

公开(公告)日：2004-08-31

申请号：US09438392

申请日：1999-11-12

申请人： Takashi Aoyama ,  Jianru Zuo ,  Nam-Hai Chua

发明人： Takashi Aoyama ,  Jianru Zuo ,  Nam-Hai Chua

IPC分类号： A01H100

CPC分类号： C12N15/8274 ,  C12N15/8209 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8242 ,  C12N15/8295

摘要： A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

摘要翻译： 描述了一种化学诱导型启动子，其可用于通过将植物或植物细胞添加到含有启动子诱导物的培养基的培养基中或通过从植物或植物细胞中除去而使其易于调节的基因转化植物，包括烟草和莴苣 这样的媒介。 所描述的启动子是可由植物内源性糖皮质激素诱导的启动子。 这样的启动子可以与各种基因如ipt或knotted1一起使用以在糖皮质激素存在下诱导芽形成。 启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导物而不是组成型的基因。 还可以列举可置于诱导型启动子控制下的基因的其他实例。

公开(公告)号：US06452068B1

公开(公告)日：2002-09-17

申请号：US09439535

申请日：1999-11-12

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N1582

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

摘要翻译： 描述了可用于通过将植物或植物细胞添加到含有启动子诱导剂的培养基的培养基中或通过从其中除去植物或植物细胞而易于调节的基因来转化植物（包括烟草和莴苣）的化学诱导型启动子 中。 所描述的启动子是由糖皮质激素或雌激素诱导的不是植物内源性的启动子。 这样的启动子可以与各种基因如ipt，CKI1或打结的1一起使用，以在合适的诱导物存在下诱导芽形成。 启动子可以与诱导体细胞胚如Lec1或SERK的基因一起使用，以制备体细胞胚，其可以生长成幼苗，然后进入植物。 启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导物而不是组成型的基因。 还可以列举可置于诱导型启动子控制下的基因的其他实例。

公开(公告)号：US20110252506A1

公开(公告)日：2011-10-13

申请号：US13164904

申请日：2011-06-21

申请人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

发明人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

IPC分类号： A01H1/00

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US20100169997A1

公开(公告)日：2010-07-01

申请号：US12722981

申请日：2010-03-12

申请人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

发明人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

IPC分类号： C12N15/82 ,  A01H4/00

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US07525012B2

公开(公告)日：2009-04-28

申请号：US10196416

申请日：2002-07-17

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  C12N5/00 ,  C12N15/63

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

公开(公告)号：US07230157B1

公开(公告)日：2007-06-12

申请号：US10129849

申请日：2000-11-13

申请人： Jianru Zuo ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Nam-Hai Chua

IPC分类号： C12N15/00 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8274 ,  C12N15/8209 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8242 ,  C12N15/8295

摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.

摘要翻译： 公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。 启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。 这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。 启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。 还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。 这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

公开(公告)号：US20120102594A1

公开(公告)日：2012-04-26

申请号：US13347839

申请日：2012-01-11

申请人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

发明人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

IPC分类号： A01H1/06

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US07816580B2

公开(公告)日：2010-10-19

申请号：US12722981

申请日：2010-03-12

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US08431773B2

公开(公告)日：2013-04-30

申请号：US13347839

申请日：2012-01-11

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.