公开(公告)号：US20090053223A1

公开(公告)日：2009-02-26

申请号：US11572116

申请日：2005-07-15

申请人： Patrick Hoffmann ,  Silke Mittelstrass ,  Jens Hennecke ,  Tobias Saum

发明人： Patrick Hoffmann ,  Silke Mittelstrass ,  Jens Hennecke ,  Tobias Saum

IPC分类号： A61K39/395 ,  C07K14/00 ,  C12P21/04 ,  A61P43/00 ,  C07K16/18

CPC分类号： C07K14/4705 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/62

摘要： A composite polypeptide, said composite polypeptide comprising a desired polypeptide and an expression enhancing domain (“EED”), said EED comprising first and second cysteine amino acid residues Cys1 and Cys2, respectively, Cys1 being located closer to the N-terminus of the composite polypeptide molecule than Cys2, wherein Cys1 and Cys2 are separated by a polypeptide linker, said linker—being free of cysteine and proline;—defining a length sufficient to allow Cys1 and Cys2 to engage in an intramolecular disulfide bond with one another; and—having a flexible polypeptide conformation essentially free of secondary polypeptide structure in aqueous solution, wherein at least one of Cys1 and Cys2 is derivatized with a derivatization moiety.

摘要翻译： 复合多肽，所述复合多肽包含期望的多肽和表达增强结构域（“EED”），所述EED分别包含第一和第二半胱氨酸氨基酸残基Cys1和Cys2，Cys1位于更靠近复合物的N末端 多肽分子，其中Cys1和Cys2被多肽接头分离，所述连接体不含半胱氨酸和脯氨酸; - 限定足以允许Cys1和Cys2彼此参与分子内二硫键的长度; 并且具有在水溶液中基本上不含次级多肽结构的柔性多肽构象，其中Cys1和Cys2中的至少一个被衍生化部分衍生化。

公开(公告)号：US08518403B2

公开(公告)日：2013-08-27

申请号：US11572116

申请日：2005-07-15

申请人： Patrick Hoffmann ,  Silke Mittelstrass ,  Jens Hennecke ,  Tobias Raum

发明人： Patrick Hoffmann ,  Silke Mittelstrass ,  Jens Hennecke ,  Tobias Raum

IPC分类号： C12P21/04 ,  C12P21/08 ,  C12N15/00 ,  C12N15/62 ,  C12N15/64 ,  A61K39/395

CPC分类号： C07K14/4705 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/62

摘要： A composite polypeptide, said composite polypeptide comprising a desired polypeptide and an expression enhancing domain (“EED”), said EED comprising first and second cysteine amino acid residues Cys1 and Cys2, respectively, Cys1 being located closer to the N-terminus of the composite polypeptide molecule than Cys2, wherein Cys1 and Cys2 are separated by a polypeptide linker, said linker—being free of cysteine and proline;—defining a length sufficient to allow Cys1 and Cys2 to engage in an intramolecular disulfide bond with one another; and—having a flexible polypeptide conformation essentially free of secondary polypeptide structure in aqueous solution, wherein at least one of Cys1 and Cys2 is derivatized with a derivatization moiety.

摘要翻译： 复合多肽，所述复合多肽包含期望的多肽和表达增强结构域（“EED”），所述EED分别包含第一和第二半胱氨酸氨基酸残基Cys1和Cys2，Cys1位于更靠近复合物的N末端 多肽分子，其中Cys1和Cys2被多肽接头分离，所述连接体不含半胱氨酸和脯氨酸; - 限定足以允许Cys1和Cys2彼此参与分子内二硫键的长度; 并且具有在水溶液中基本上不含次级多肽结构的柔性多肽构象，其中Cys1和Cys2中的至少一个被衍生化部分衍生化。