专利检索 ap:"Instytut Biologii Doświadczalnej im. Marcelego Nenckiego Polska Akademia Nauk" 第 1 页

1.

发明授权
Genetically encoded FRET-based MMP-9 activity biosensor and use thereof 有权

公开(公告)号：US09914955B2

公开(公告)日：2018-03-13

申请号：US14911821

申请日：2014-08-12

申请人： Instytut Biologii Doświadczalnej im. Marcelego Nenckiego Polska Akademia Nauk

发明人： Michal Stawarski , Jakub Wlodarczyk , Leszek Kaczmarek

IPC分类号： A61K39/00 , C07K14/00 , C07K16/00 , C12Q1/37 , G01N33/542

CPC分类号： C12Q1/37 , C07K14/00 , C07K2319/50 , C07K2319/60 , G01N33/542 , G01N2333/96494

摘要： The present invention relates to a genetically encoded FRET-based biosensor to monitor the activity of matrix metalloproteinase 9 (MMP-9). MMP-9 is an extracellular acting endopeptidase implicated in both physiological and pathological processes. A genetically encoded FRET biosensor anchored in the cellular membrane allows studying the proteolytic activity of MMP-9 with high spatiotemporal resolution at the exact region of MMP-9 action on the cell. Applicability of the biosensor, both in vitro and in vivo in living cells, has been demonstrated by ratiometric analysis of cleavage of the biosensor by a purified auto-activating mutant of MMP-9.