公开(公告)号：US20080153125A1

公开(公告)日：2008-06-26

申请号：US11937992

申请日：2007-11-09

Applicant: Daniel A. BUTTRY ,  Angela M. OPPEDAHL ,  Robert CZARNEK ,  Martin C. FOSTER ,  Steven J. LASKY ,  Ho-Ming PANG

Inventor： Daniel A. BUTTRY ,  Angela M. OPPEDAHL ,  Robert CZARNEK ,  Martin C. FOSTER ,  Steven J. LASKY ,  Ho-Ming PANG

IPC: C12Q1/24 ,  C12M1/34 ,  C12M1/00

CPC classification number: C12M23/12 ,  C12M23/22 ,  C12M25/04 ,  C12M41/36 ,  C12Q1/04

Abstract: A system for the rapid detection of microbial contamination in a fluid sample such as water, involving the use of a filter material having a surface adapted to receive the sample in order to retain substantially all microbes from the sample on the filter surface under conditions that minimize the potential for contamination from sources other than the sample itself, and in a manner that permits the filter surface to be incubated in order to grow viable microbes contained thereon, in combination with a growth medium and an analytic instrument to permit analysis of the filter surface, within a predetermined incubation period, in order to determine whether the growth onset of viable microbes that may be present on the surface has begun.

Abstract translation: 一种用于快速检测诸如水的流体样品中的微生物污染的系统，其涉及使用具有适于接收样品的表面的过滤材料，以便在最小化条件下将来自样品的基本上所有微生物从样品保持在过滤表面上 来自除了样品本身之外的来源的污染的可能性，并且以允许过滤表面孵育以便生长包含在其上的活的微生物的方式与生长培养基和分析仪器组合以允许分析过滤表面 在预定的潜伏期内，以确定是否开始可能存在于表面上的活微生物的生长发生。

公开(公告)号：US08101353B2

公开(公告)日：2012-01-24

申请号：US12338373

申请日：2008-12-18

Applicant: Gary W. Procop ,  Wei Wei ,  Ho-ming Pang

Inventor： Gary W. Procop ,  Wei Wei ,  Ho-ming Pang

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2537/157 ,  C12Q2523/107

Abstract: The invention includes a method of sample profiling for identification. The method includes the steps of performing less than four nucleotide-specific chemical cleavage reactions to obtain nucleotide sequence fragments, performing size separation on the fragments, detecting the fragments' separation, generating a profile based on the detection, and comparing the profile to a data base to identify the sample.

Abstract translation: 本发明包括用于鉴定的样品分析方法。 该方法包括以下步骤：进行少于四个核苷酸特异性化学切割反应以获得核苷酸序列片段，在片段上进行大小分离，检测片段的分离，基于检测产生轮廓，并将轮廓与数据进行比较 用于识别样品。

公开(公告)号：US20110068007A1

公开(公告)日：2011-03-24

申请号：US12883475

申请日：2010-09-16

Applicant: HO-MING PANG ,  WEI WEI

Inventor： HO-MING PANG ,  WEI WEI

IPC: G01N27/447

CPC classification number: G01N27/44721

Abstract: Using multiple dichroics mirrors to split and redirect different wavelength regions of light in a multi-wavelength fluorescence detection system.

Abstract translation: 使用多个二向色镜在多波长荧光检测系统中分离和重定向不同波长的光区域。

公开(公告)号：US07497937B2

公开(公告)日：2009-03-03

申请号：US10934259

申请日：2004-09-03

Applicant: Edward S. Yeung ,  Ho-Ming Pang ,  Futian Han

Inventor： Edward S. Yeung ,  Ho-Ming Pang ,  Futian Han

IPC: G01N27/453

CPC classification number: G01N27/44721 ,  C07K1/26 ,  G01N27/44791

Abstract: A multiplexed, absorbance-based microfabricated chip electrophoresis system, microfabricated chip, and method of use are included for the detection and identification of protein species. The microfabricated chip electrophoresis system capable of analyzing multiple samples simultaneously. The system uses a microfabricated chip having a sample chamber for first dimension separation of a sample, a first planar array of channels for second dimension separation of the sample approximately perpendicular the sample chamber, and a barrier separating the sample chamber from the first planar array of channels during the first dimension separation. In use, the sample is placed into a sample chamber, a barrier is established around the sample chamber, the sample is isoelectric focused within the sample chamber, the barrier is then removed, and the sample separated in the first array of channels by molecular weight.

Abstract translation: 包含复合的，以吸光度为基础的微芯片电泳系统，微加工芯片和使用方法用于检测和鉴定蛋白质。 该微电解芯片电泳系统能够同时分析多个样品。 该系统使用具有样品室的微制造芯片，用于样品的第一维分离，用于样品大致垂直于样品室的第二维分离的通道的第一平面阵列，以及将样品室与第一平面阵列 通道在第一维分离期间。 在使用中，将样品放置在样品室中，在样品室周围建立屏障，样品在样品室内等电聚焦，然后除去屏障，样品在第一阵列通道中以分子量分离 。

公开(公告)号：US08357281B2

公开(公告)日：2013-01-22

申请号：US12883475

申请日：2010-09-16

Applicant: Ho-Ming Pang ,  Wei Wei

Inventor： Ho-Ming Pang ,  Wei Wei

IPC: G01N27/00

CPC classification number: G01N27/44721

Abstract: Using multiple dichroics mirrors to split and redirect different wavelength regions of light in a multi-wavelength fluorescence detection system.

Abstract translation: 使用多个二向色镜在多波长荧光检测系统中分离和重定向不同波长的光区域。

公开(公告)号：US20110120869A1

公开(公告)日：2011-05-26

申请号：US13012446

申请日：2011-01-24

Applicant: HO-MING PANG

Inventor： HO-MING PANG

IPC: G01N27/447

CPC classification number: B01D57/02 ,  G01N27/44782

Abstract: The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal.

Abstract translation: 本发明提供了在数据生成之后校正串扰的简单方法。 通过重复的过程简单地从原始观察信号中减去相邻的信号。 当满足预定义条件时，停止数据处理。 通过这种技术，串扰可以从> 5％降低到小于0.1％。 而且作为另外的优点，该方法提供了一种校正串扰的方法，而不需要知道相邻毛细管信号引起哪些峰。

公开(公告)号：US07901557B2

公开(公告)日：2011-03-08

申请号：US12426317

申请日：2009-04-20

Applicant: Ho-Ming Pang

Inventor： Ho-Ming Pang

IPC: G01N27/447

CPC classification number: B01D57/02 ,  G01N27/44773

Abstract: The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal.

Abstract translation: 本发明提供了在数据生成之后校正串扰的简单方法。 通过重复的过程简单地从原始观察信号中减去相邻的信号。 当满足预定义条件时，停止数据处理。 通过这种技术，串扰可以从> 5％降低到小于0.1％。 而且作为另外的优点，该方法提供了一种校正串扰的方法，而不需要知道相邻毛细管信号引起哪些峰值。

公开(公告)号：US20100264028A1

公开(公告)日：2010-10-21

申请号：US12426317

申请日：2009-04-20

Applicant: HO-MING PANG

Inventor： HO-MING PANG

IPC: B01D57/02

CPC classification number: B01D57/02 ,  G01N27/44773

Abstract: The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal.

Abstract translation: 本发明提供了在数据生成之后校正串扰的简单方法。 通过重复的过程简单地从原始观察信号中减去相邻的信号。 当满足预定义条件时，停止数据处理。 通过这种技术，串扰可以从> 5％降低到小于0.1％。 而且作为另外的优点，该方法提供了一种校正串扰的方法，而不需要知道相邻毛细管信号引起哪些峰。

公开(公告)号：US20100140505A1

公开(公告)日：2010-06-10

申请号：US12634349

申请日：2009-12-09

Applicant: Ho-ming Pang ,  Wei Wei

Inventor： Ho-ming Pang ,  Wei Wei

IPC: G01J1/58

CPC classification number: G01N27/44791 ,  G01N21/6428 ,  G01N27/44721 ,  G01N27/44726

Abstract: The invention includes a high sensitivity and high throughput capillary electrophoresis multiwavelength florescence detection system. The fluorescent detection system is configured to illuminate a relatively large volume of a single capillary or a plurality of capillaries, with a pixelated detection system capable of imaging an area of each capillary that differentiates the capillary walls, the space between the capillaries, and the internal liquid volume within the capillary. Only the desired pixels or image area are used for processing and generating an output signal.

Abstract translation: 本发明包括高灵敏度和高通量的毛细管电泳多波长荧光检测系统。 荧光检测系统被配置为照亮相对较大体积的单个毛细管或多个毛细管，其中像素化检测系统能够对每个毛细管的区域进行成像，以区分毛细管壁，毛细管之间的空间和内部 毛细管内的液体体积。 仅使用期望的像素或图像区域来处理和产生输出信号。

公开(公告)号：US06969452B2

公开(公告)日：2005-11-29

申请号：US10377909

申请日：2003-02-28

Applicant: Yan He ,  Ho-Ming Pang ,  Siquan Luo ,  Futian Han

Inventor： Yan He ,  Ho-Ming Pang ,  Siquan Luo ,  Futian Han

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44773

Abstract: Disclosed is a two-dimensional protein separation method. It makes separating a protein sample by chromatofocusing into a plurality of aliquots, and then loading each aliquot into a separate capillary tube; and separating each aliquot by multiplexed capillary electrophoresis to produce a two-dimensional array of separated proteins. A preferred integrated buffer for this system is also disclosed.

Abstract translation: 公开了二维蛋白质分离方法。 它使蛋白质样品通过色谱聚焦分成多个等分试样，然后将每个等分试样装载到单独的毛细管中; 并通过多重毛细管电泳分离每个等分试样以产生分离的蛋白质的二维阵列。 还公开了该系统的优选集成缓冲器。