公开(公告)号：US20230130635A1

公开(公告)日：2023-04-27

申请号：US18085751

申请日：2022-12-21

申请人： HELMHOLTZ ZENTRUM MUNCHEN - DEUTSCHES FORSCHUNGSZE ,  SIRION BIOTECH GMBH

发明人： Natasa ANASTASOV ,  Ines HOFIG ,  Christian THIRION

IPC分类号： C12N15/86 ,  C12N15/867 ,  A61K48/00

摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.