公开(公告)号：US09834753B2

公开(公告)日：2017-12-05

申请号：US14367813

申请日：2012-12-18

Applicant: Mogam Biotechnology Institute ,  Green Cross Labcell

Inventor： Bokyung Min ,  Hana Choi ,  Okjae Lim ,  Jung Hyun Her ,  Sangmi Kang ,  Eun-Kyoung Lee ,  Hyejin Chung ,  Yu Kyeong Hwang

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/18 ,  C12N5/0783 ,  A61K35/17 ,  A61K38/38

CPC classification number: C12N5/0646 ,  A61K35/17 ,  A61K38/38 ,  C12N2501/2302 ,  C12N2501/515

Abstract: The present invention relates to a method for producing natural killer cells (hereinafter, referred to as “NK cells”), NK cells produced thereby, and a composition for treating cancers and infectious diseases containing the same. The present invention provides a method for producing NK cells, which maintain high cytotoxicity and cell viability to exhibit high therapeutic effects against cancers and infectious diseases and can be cultured ex vivo at high efficiency and high concentration. In addition, a culture method which maintains cell concentration at a constant level is used for the production of NK cells, and thus the overgrowth of the cells can be prevented so that the cells can be maintained at an optimal state. Particularly, even when the cells are thawed after freezing, the function of the cells is not impaired, and the NK cells can maintain high cell viability and cytotoxicity. Thus, the NK cells can be easily stored and supplied in a liquid or frozen state without needing an additional treatment process.

公开(公告)号：US20150118207A1

公开(公告)日：2015-04-30

申请号：US14367813

申请日：2012-12-18

Applicant: Mogam Biotechnology Institute ,  Green Cross Labcell

Inventor： Bokyung Min ,  Hana Choi ,  Okjae Lim ,  Jung Hyun Her ,  Sangmi Kang ,  Eun-Kyoung Lee ,  Hyejin Chung ,  Yu Kyeong Hwang

IPC: C12N5/0783 ,  A61K38/38 ,  A61K35/17

CPC classification number: C12N5/0646 ,  A61K35/17 ,  A61K38/38 ,  C12N2501/2302 ,  C12N2501/515

Abstract: The present invention relates to a method for producing natural killer cells (hereinafter, referred to as “NK cells”), NK cells produced thereby, and a composition for treating cancers and infectious diseases containing the same. The present invention provides a method for producing NK cells, which maintain high cytotoxicity and cell viability to exhibit high therapeutic effects against cancers and infectious diseases and can be cultured ex vivo at high efficiency and high concentration. In addition, a culture method which maintains cell concentration at a constant level is used for the production of NK cells, and thus the overgrowth of the cells can be prevented so that the cells can be maintained at an optimal state. Particularly, even when the cells are thawed after freezing, the function of the cells is not impaired, and the NK cells can maintain high cell viability and cytotoxicity. Thus, the NK cells can be easily stored and supplied in a liquid or frozen state without needing an additional treatment process.

Abstract translation: 本发明涉及一种生产天然杀伤细胞的方法（以下称为“NK细胞”），由此产生的NK细胞，以及用于治疗癌症的组合物和含有该NK细胞的感染性疾病。 本发明提供一种生产NK细胞的方法，其保持高细胞毒性和细胞活力，对癌症和感染性疾病表现出高疗效，并可以以高效率和高浓度离体培养。 此外，将细胞浓度维持在一定水平的培养方法用于NK细胞的产生，因此可以防止细胞的过度生长，使细胞保持在最佳状态。 特别是，即使细胞在冷冻后解冻，也不会损害细胞的功能，NK细胞可维持高的细胞活力和细胞毒性。 因此，NK细胞可以容易地以液体或冷冻状态储存和供应，而不需要额外的处理过程。