公开(公告)号：US08192931B2

公开(公告)日：2012-06-05

申请号：US12500749

申请日：2009-07-10

申请人： Yves Fradet ,  Camille Chypre ,  Lyson Piché ,  Geneviéve Garon

发明人： Yves Fradet ,  Camille Chypre ,  Lyson Piché ,  Geneviéve Garon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16

摘要： The present invention relates to prostate cancer. More specifically, the present invention relates to a method to detect prostate cancer in a patient sample by detecting the RNA encoded by the gene PCA3. More particularly the present invention relates to a method for determining a predisposition, or presence of prostate cancer in a patient comprising: (a) contacting a biological sample of a patient with at least one oligonucleotide that hybridizes to a PCA3 polynucleotide; (b) detecting in the biological sample an amount of PCA3 and second prostate specific polynucleotides; and (c) comparing the amount of PCA3 polynucleotide that hybridizes to the oligonucleotide to a predetermined cut off value, and therefrom determining the presence or absence of prostate cancer in the biological sample. The present invention further relates to diagnostic kits for the detection of prostate cancer or the risk of developing same in a patient comprising: (a) at least one container means having disposed therein at least one oligonucleotide probe or primer that hybridizes to one a PCA3 nucleic acid or complement thereof; (b) at least one oligonucleotide probe or primer that hybridizes with a second prostate specific nucleic acid or complement thereof; and (c) reagents enabling a detection of PCA3 and of the second prostate specific nucleic acid when PCA3 or second prostate-specific nucleic acid sequence is present.

摘要翻译： 本发明涉及前列腺癌。 更具体地，本发明涉及通过检测由基因PCA3编码的RNA来检测患者样品中前列腺癌的方法。 更具体地说，本发明涉及一种用于确定患者中前列腺癌的倾向或存在的方法，包括：（a）使患者的生物样品与至少一种与PCA3多核苷酸杂交的寡核苷酸接触; （b）在生物样品中检测PCA3和第二前列腺特异性多核苷酸的量; 和（c）将与寡核苷酸杂交的PCA3多核苷酸的量与预定的切断值进行比较，从而确定生物样品中前列腺癌的存在或不存在。 本发明还涉及用于检测前列腺癌的诊断试剂盒或在患者中发生相同危险的诊断试剂盒，其包含：（a）至少一个容器装置，其中设置有至少一种与PCA3核酸之一杂交的寡核苷酸探针或引物 酸或其互补物; （b）与第二前列腺特异性核酸或其互补体杂交的至少一种寡核苷酸探针或引物; 和（c）当存在PCA3或第二前列腺特异性核酸序列时能够检测PCA3和第二前列腺特异性核酸的试剂。