公开(公告)号：US06660494B2

公开(公告)日：2003-12-09

申请号：US10021271

申请日：2001-12-19

申请人： Günter Schabert ,  Lawrence Restaino ,  Elon W. Frampton

发明人： Günter Schabert ,  Lawrence Restaino ,  Elon W. Frampton

IPC分类号： C12Q104

CPC分类号： C07F9/5728 ,  C12Q1/04 ,  C12Q1/44 ,  G01N2333/916 ,  Y10S435/975

摘要： Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R1, R2, R3, and R4 are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

摘要翻译： 式（I）的显色3-吲哚基胆碱磷酸酯化合物：其中R选自氢和C 1-4烷基，例如甲基，乙基，丙基和丁基，而R 1，R 2，R R 3独立地选自氢，卤素，氰基，硝基，羧基，氨基，被一个或两个C 1-4烷基取代的氨基，氨基甲基，羟基，C 1-4烷氧基， 羧基烷基和磺酰基。 这些化合物能够被卵磷脂酶C裂解，导致可量热检测的产物。 本发明提供了安全和敏感的检测，例如产气荚膜梭菌，蜡状芽孢杆菌，炭疽芽孢杆菌，铜绿假单胞菌，单核细胞增生利斯特氏菌，幽门螺杆菌，嗜肺军团菌等可能具有这种活性的细菌活性，其中可能含有通常包括生理样品 消费品，如食品和饮料，以及任何其他可能感染的物体或物品。