DNA CONSTRUCTS COMPRISING ALTERNATIVE PROMOTERS

    公开(公告)号:US20240209357A1

    公开(公告)日:2024-06-27

    申请号:US18039578

    申请日:2021-12-01

    申请人: ETH ZÜRICH

    IPC分类号: C12N15/113 C12N15/86

    摘要: The invention relates to the fields of biomolecular computing and/or synthetic biology. In particular, the invention relates to a DNA construct that comprises a first promoter, at least one further promoter and an output sequence, wherein each of said promoters comprises a transcription start site and/or is suitable for initiating transcription, wherein the initiation of transcription from the first promoter is enabled by a first transcriptional regulatory state and the initiation of transcription from each of said further promoter(s) is enabled by a respective further transcriptional regulatory state, and wherein said DNA construct yields an effective amount of an output RNA in a eukaryotic cell, when said first transcriptional regulatory state and/or any of the respective further transcriptional regulatory states is present in said cell, wherein said output RNA comprises a sequence corresponding to said output sequence. Furthermore, the invention relates to medical and/or diagnostic uses of the inventive DNA construct of the invention, e.g., for detecting, killing and/or manipulating different types of eukaryotic target cells in a subject and/or in a tissue sample.

    Electrical converter and method for operating an electrical converter

    公开(公告)号:US20240171138A1

    公开(公告)日:2024-05-23

    申请号:US18263140

    申请日:2022-02-03

    申请人: ETH Zürich

    IPC分类号: H03F3/217 H02M3/338

    CPC分类号: H03F3/2178 H02M3/338

    摘要: A DC-DC converter topology based on electromagnetically coupled class-D LC oscillator is proposed. An electrical converter comprises at least two oscillators (1,2), each of the at least two oscillators being designed to have an oscillating current and an oscillating voltage. Coupling elements (16, 17, 18, 19) arranged to couple the oscillating currents of the at least two oscillators and/or the oscillating voltages of the at least two oscillator. The at least two oscillators are connected in a series connection, adding their oscillating voltages, and/or in a parallel connection, adding their oscillating currents. The topology can be fully integrated, that is, it can be realized as an integrated circuit without external components, in particular without external passive components, such as capacitors and/or inductors.