公开(公告)号：US06331524B1

公开(公告)日：2001-12-18

申请号：US08835213

申请日：1997-04-07

申请人： Ronald K. Scheule ,  Rebecca G. Bagley ,  Simon J. Eastman ,  Seng H. Cheng ,  John Marshall ,  David J. Harris ,  Edward R. Lee ,  Craig S. Siegel ,  Chau-Dung Chang ,  S. Catherine Hubbard ,  Duane E. Johnson ,  Daniel C. Maneval ,  H. Michael Shepard ,  Richard J. Gregory

发明人： Ronald K. Scheule ,  Rebecca G. Bagley ,  Simon J. Eastman ,  Seng H. Cheng ,  John Marshall ,  David J. Harris ,  Edward R. Lee ,  Craig S. Siegel ,  Chau-Dung Chang ,  S. Catherine Hubbard ,  Duane E. Johnson ,  Daniel C. Maneval ,  H. Michael Shepard ,  Richard J. Gregory

IPC分类号： A61K4800

CPC分类号： C07J41/0055 ,  A61K9/1272 ,  A61K47/54 ,  A61K47/542 ,  A61K47/543 ,  A61K47/554 ,  A61K48/00 ,  C07J41/0005 ,  C07J41/0061 ,  C07J43/003 ,  C12N15/88

摘要： Novel cationic amphiphiles are provided that facilitate transport of biologically active (therapeutic) molecules into cells. The amphiphiles contain lipophilic groups derived from steroids, from mono or dialkylamines, or from alkyl or acyl groups; and cationic groups, protonatable at physiological pH, derived from amines, alkylamines or polyalkylamines. There are provided also therapeutic compositions prepared typically by contacting a dispersion of one or more cationic amphiphiles with the therapeutic molecules. Therapeutic molecules that can be delivered into cells according to the practice of the invention include DNA, RNA, and polypeptides. Representative uses of the therapeutic compositions of the invention include providing gene therapy, and delivery of antisense polynucleotides or biologically active polypeptides to cells. With respect to therapeutic compositions for gene therapy, the DNA is provided typically in the form of a plasmid for complexing with the cationic amphiphile. Novel and highly effective plasmid constructs are also disclosed, including those that are particularly effective at providing gene therapy for clinical conditions complicated by inflammation. Additionally, targeting of organs for gene therapy by intravenous administration of therapeutic compositions is described.

摘要翻译： 提供了新的阳离子两亲物，其促进生物活性（治疗性）分子进入细胞。 两亲物含有衍生自类固醇的单亲或二烷基胺或烷基或酰基的亲脂基团; 和在生理pH下可质子化的阳离子基团，衍生自胺，烷基胺或多烷基胺。 还提供了通常通过使一种或多种阳离子两亲物的分散体与治疗分子接触而制备的治疗组合物。 根据本发明的实践可以递送到细胞中的治疗分子包括DNA，RNA和多肽。 本发明治疗组合物的代表性用途包括提供基因治疗以及向细胞递送反义多核苷酸或生物活性多肽。 关于基因治疗的治疗组合物，通常以与阳离子两亲物络合的质粒的形式提供DNA。 还公开了新型和高效的质粒构建体，包括在为炎症复杂的临床病症提供基因治疗方面特别有效的质粒构建体。 另外，描述了通过静脉内施用治疗组合物靶向用于基因治疗的器官。

公开(公告)号：US3996393A

公开(公告)日：1976-12-07

申请号：US454058

申请日：1974-03-25

申请人： Charles A. Cortellino ,  Edward Gipstein ,  William A. Hewett ,  Duane E. Johnson ,  Wayne M. Moreau

发明人： Charles A. Cortellino ,  Edward Gipstein ,  William A. Hewett ,  Duane E. Johnson ,  Wayne M. Moreau

IPC分类号： G03F7/039 ,  H01L21/027 ,  B05D3/06

CPC分类号： G03F7/039

摘要： It has been discovered that the sensitivity of positive acting polymeric electron beam resists is increased by well over an order of magnitude by using polymers having a molecular weight of at least one million, thereby making it possible to reduce the required radiation to below 3 .times. 10.sup..sup.-6 coulombs/cm.sup.2.

摘要翻译： 已经发现，通过使用具有至少一百万分子量的聚合物，正性聚合物电子束抗蚀剂的敏感性提高了一个数量级以上，从而使得所需辐射降至3×10 6库伦/ cm2。

公开(公告)号：US4074031A

公开(公告)日：1978-02-14

申请号：US721259

申请日：1976-09-08

申请人： Duane E. Johnson ,  Lester A. Pederson

发明人： Duane E. Johnson ,  Lester A. Pederson

IPC分类号： G03F7/20 ,  G03C1/72 ,  G03C5/08 ,  G03F7/039 ,  H01L21/027 ,  C08F8/32

CPC分类号： G03F7/039

摘要： Positive electron beam resists are prepared by reacting a film which is a copolymer of methyl methacrylate and methacrylic acid with a tertiary amine.

摘要翻译： 通过使作为甲基丙烯酸甲酯和甲基丙烯酸的共聚物的膜与叔胺反应来制备正电子束抗蚀剂。