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公开(公告)号：US20080317719A1
公开(公告)日：2008-12-25
申请号：US11820975
申请日：2007-06-20
CPC分类号： C12N5/0647 , C12N2501/165 , C12N2501/91
摘要： A method is provided, including in vitro stimulating an initiating cell population (ICP) of at least 5 million cells that have a density of less than 1.072 g/ml, and at least 1% of which are CD34+CD45−/dim, to differentiate into a progenitor/precursor cell population (PCP). A method is provided, including in vitro stimulating an initiating cell population (ICP) of at least ten thousand cells that have a density of less than 1.072 g/ml to differentiate into a progenitor/precursor cell population (PCP). A method is provided, including separating lower density cells from higher density cells, the lower density cells defining an initiating cell population (ICP), and in vitro stimulating the ICP to differentiate into a progenitor/precursor cell population (PCP). Other embodiments are also described.
摘要翻译： 提供了一种方法，包括体外刺激至少500万个细胞的起始细胞群（ICP），其具有小于1.072g / ml的密度，并且其中至少1％是CD34 + CD45- / dim，至 分化成祖细胞/前体细胞群（PCP）。 提供了一种方法，包括体外刺激至少一万个细胞的起始细胞群（ICP），其具有小于1.072g / ml的密度以分化为祖细胞群（PCP）。 提供了一种方法，包括从较高密度细胞分离较低密度的细胞，较低密度的细胞限定起始细胞群（ICP），并体外刺激ICP分化成祖细胞/前体细胞群（PCP）。 还描述了其它实施例。
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公开(公告)号：US09404084B2
公开(公告)日：2016-08-02
申请号：US11820975
申请日：2007-06-20
IPC分类号： C12N5/00 , C12N5/0789
CPC分类号： C12N5/0647 , C12N2501/165 , C12N2501/91
摘要： A method is provided, including in vitro stimulating an initiating cell population (ICP) of at least 5 million cells that have a density of less than 1.072 g/ml, and at least 1% of which are CD34+CD45−/dim, to differentiate into a progenitor/precursor cell population (PCP). A method is provided, including in vitro stimulating an initiating cell population (ICP) of at least ten thousand cells that have a density of less than 1.072 g/ml to differentiate into a progenitor/precursor cell population (PCP). A method is provided, including separating lower density cells from higher density cells, the lower density cells defining an initiating cell population (ICP), and in vitro stimulating the ICP to differentiate into a progenitor/precursor cell population (PCP). Other embodiments are also described.
摘要翻译： 提供了一种方法，包括体外刺激至少500万个细胞的起始细胞群（ICP），其具有小于1.072g / ml的密度，并且其中至少1％是CD34 + CD45- / dim，至 分化成祖细胞/前体细胞群（PCP）。 提供了一种方法，包括体外刺激至少一万个细胞的起始细胞群（ICP），其具有小于1.072g / ml的密度以分化为祖细胞群（PCP）。 提供了一种方法，包括从较高密度细胞分离较低密度的细胞，较低密度的细胞限定起始细胞群（ICP），并体外刺激ICP分化成祖细胞/前体细胞群（PCP）。 还描述了其它实施例。
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公开(公告)号：US08685724B2
公开(公告)日：2014-04-01
申请号：US11628488
申请日：2005-06-01
IPC分类号： C12N15/00
CPC分类号： C12N5/0692 , C12N5/0634 , C12N2501/165 , C12N2506/11
摘要： A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g/ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.
摘要翻译： 提供了一种与提取的血液一起使用的方法，其包括（a）将血液施用于适合于选择密度小于1.077g / ml的先前细胞的第一梯度; （b）将第一遍细胞施用于适合于选择密度在1.055和1.074g / ml之间的第二次细胞的第二梯度; （c）通过培养二代细胞持续3至30天的时间，增加密度在1.055和1.074g / ml之间的细胞数; 和（d）鉴定培养细胞中的内皮祖细胞。 还描述了其它实施例。
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公开(公告)号：US20080220466A1
公开(公告)日：2008-09-11
申请号：US11628488
申请日：2005-06-01
IPC分类号： C12Q1/04
CPC分类号： C12N5/0692 , C12N5/0634 , C12N2501/165 , C12N2506/11
摘要： A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g/ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.
摘要翻译： 提供了一种与提取的血液一起使用的方法，其包括（a）将血液施用于适合于选择密度小于1.077g / ml的先前细胞的第一梯度; （b）将第一遍细胞施用于适合于选择密度在1.055和1.074g / ml之间的第二次细胞的第二梯度; （c）通过培养二代细胞持续3至30天的时间，增加密度在1.055和1.074g / ml之间的细胞数; 和（d）鉴定培养细胞中的内皮祖细胞。 还描述了其它实施例。
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