公开(公告)号：US5264555A

公开(公告)日：1993-11-23

申请号：US913138

申请日：1992-07-14

申请人： Robert G. L. Shorr ,  Kwang Nho ,  Myung-ok P. Cho ,  Chyi Lee ,  Barbara Czuba ,  Hariharan Shankar

发明人： Robert G. L. Shorr ,  Kwang Nho ,  Myung-ok P. Cho ,  Chyi Lee ,  Barbara Czuba ,  Hariharan Shankar

IPC分类号： C07K14/805 ,  C07K3/02 ,  C07K3/12 ,  C07K3/22 ,  C07K15/06

CPC分类号： C07K14/805

摘要： Methods are disclosed for separating hemoglobin from erythrocytes by contacting erythrocytes with a hypotonic buffer solution at a rate sufficient to render the release of hemoglobin from said erythrocytes without significant lysis. The hemoglobin is then separated from the erythrocytes. Methods are also disclosed for purifying hemoglobin solutions of DNA, endotoxins and phospholipids by contacting the hemoglobin solutions with an anion exchange medium.

摘要翻译： 公开了通过使红细胞与低渗缓冲溶液接触的速率，以足以使血红蛋白从所述红细胞中释放而没有显着裂解的速率来分离血红蛋白与红细胞的方法。 然后将血红蛋白与红细胞分离。 还公开了通过使血红蛋白溶液与阴离子交换介质接触来纯化DNA，内毒素和磷脂的血红蛋白溶液的方法。