公开(公告)号：US08911937B2

公开(公告)日：2014-12-16

申请号：US12219378

申请日：2008-07-21

申请人： Tomasz Kazimierz Wojdacz ,  Lise Lotte Hansen ,  Alexander Dobrovic

发明人： Tomasz Kazimierz Wojdacz ,  Lise Lotte Hansen ,  Alexander Dobrovic

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/185 ,  C12Q2523/125

摘要： A reliable and highly sensitive method is provided for detecting methylation status of CpG-containing nucleic acids by nucleic acid amplification and melting curve analysis of amplification products. The methods and compositions employs a novel design of primers, CpG-containing methylation-independent oligonucleotide primers, wherein both unmethylated and methylated alleles of a CpG-containing nucleic acid can be detected by use of only one set of primers after the CpG-containing nucleic acid has been subjected to cytosine to thymine conversion of unmethylated Cytosine. The method is useful for detection of methylation status in for example cancer genes and other disease related genes, wherein methylation influences gene expression.

摘要翻译： 通过核酸扩增和扩增产物的融合曲线分析，提供了一种可靠且高度灵敏的检测含CpG的核酸甲基化状态的方法。 该方法和组合物采用引物，含CpG的甲基化独立的寡核苷酸引物的新颖设计，其中含CpG的核酸的非甲基化和甲基化等位基因都可以在含有CpG的核酸之后仅使用一组引物来检测 酸已经被胞嘧啶转化成未甲基化的胞嘧啶的胸腺嘧啶转化。 该方法可用于检测例如癌基因和其他疾病相关基因中的甲基化状态，其中甲基化影响基因表达。

公开(公告)号：US08634614B2

公开(公告)日：2014-01-21

申请号：US12995770

申请日：2009-06-02

申请人： Jamila Ahdidan Madsen

发明人： Jamila Ahdidan Madsen

IPC分类号： G06K9/00

CPC分类号： G06T7/0012 ,  G06T7/41 ,  G06T7/62 ,  G06T2207/10016 ,  G06T2207/10088 ,  G06T2207/20076 ,  G06T2207/20128 ,  G06T2207/30016

摘要： The invention relates to a system and a method for analysis and evaluation of at least one anatomical structure in a medical image, said medical image representing at least a part of a patient or person, said system comprising image analyzing means for calculating the volume and/or providing the shape of the at least one anatomical structure, and statistical analyzing means for providing a confidence interval of the volume and/or the shape of the analyzed anatomical structure(s) based on individual data of the patient or person, thereby quantifying the normality of said anatomical structure(s). By the present system and method a detailed and trustworthy confidence interval can be provided in connection with analysis and evaluation of a medical image of a patient, thereby illustrating the expected normality of the patient. The invention can be applied to images acquired from magnetic resonance imaging (MRI). One example of use is for diagnosing diseases and/or initial stages of diseases.

摘要翻译： 本发明涉及一种用于分析和评估医学图像中的至少一个解剖结构的系统和方法，所述医学图像表示患者或个人的至少一部分，所述系统包括图像分析装置，用于计算体积和/ 或提供所述至少一个解剖结构的形状，以及统计分析装置，用于基于患者或个人的个体数据提供所分析的解剖结构的体积和/或形状的置信区间，从而量化 所述解剖结构的正常性。 通过本系统和方法，可以结合对患者的医学图像的分析和评估提供详细且可靠的置信区间，从而说明患者的预期的正常性。 本发明可以应用于从磁共振成像（MRI）获得的图像。 使用的一个例子是诊断疾病和/或疾病的初始阶段。