公开(公告)号：US20230349008A1

公开(公告)日：2023-11-02

申请号：US18025965

申请日：2021-09-15

申请人： CORNELL UNIVERSITY

发明人： Ruisheng WANG ,  David ERICKSON

IPC分类号： C12Q1/70 ,  C12Q1/6844 ,  B01L3/00

CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  B01L3/5023 ,  B01L2300/165 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/126

摘要： A sample testing chip includes a first layer formed of a porous hydrophilic material. One or more hydrophobic barriers are located in the first layer to define one or more testing areas configured to receive a volume of a sample and one or more auxiliary areas. The one or more testing areas and the one or more auxiliary areas are separated from one another by the hydrophobic barrier and are not fluidically connected. Methods of fabrication and use of the sample testing chip are also disclosed.

公开(公告)号：US11795498B2

公开(公告)日：2023-10-24

申请号：US18170285

申请日：2023-02-16

申请人： 10x Genomics Sweden AB

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6844 ,  G16B50/30 ,  G16B50/20 ,  G16B30/00 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q1/682 ,  G01N1/30 ,  G01N1/42

CPC分类号： C12Q1/6837 ,  C12N15/1065 ,  C12Q1/682 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30 ,  G01N1/42 ,  G16B30/00 ,  G16B50/20 ,  G16B50/30 ,  C12Y600/00 ,  C12Q1/6837 ,  C12Q2543/101 ,  C12Q2565/514 ,  C12Q2565/537 ,  C12Q1/6841 ,  C12Q2543/101 ,  C12Q2565/514 ,  C12Q2565/537 ,  C12Q1/6844 ,  C12Q2543/101 ,  C12Q2565/514 ,  C12Q2565/537

摘要： Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20230332254A1

公开(公告)日：2023-10-19

申请号：US17881008

申请日：2022-08-04

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Walter Ian Lipkin ,  Thomas BRIESE ,  Nischay MISHRA ,  Rafal TOKARZ ,  Simon H. WILLIAMS

IPC分类号： C12Q1/70 ,  C12Q1/6844

CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  C12Q2600/16

摘要： Disclosed here are compositions and methods for the detection of at least one virus viruses using a one-step assay. The viruses to be detected include at least SARS-CoV-2 and influenza viruses. In particular, the disclosure relates to a method of detection of the viruses using specific primers and probes designed to detect and if necessary differentiate between the viruses.

公开(公告)号：US11773426B2

公开(公告)日：2023-10-03

申请号：US16514814

申请日：2019-07-17

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： David Joun ,  Chieh-Yuan Li ,  Brian Reed ,  Craig Obergfell ,  Devin Dressman ,  Abraham Rosenbaum ,  Scott Benson ,  Andi Broka ,  Srinka Ghosh

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q1/6844 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537

摘要： In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

公开(公告)号：US20230295719A1

公开(公告)日：2023-09-21

申请号：US18184556

申请日：2023-03-15

申请人： ILLUMINA, INC.

发明人： Aathavan Karunakaran ,  Nileshi Saraf ,  Samantha Antonio Leong ,  Ramir Villa Vega ,  Gery Vessere

IPC分类号： C12Q1/6874 ,  C12Q1/6844

CPC分类号： C12Q1/6874 ,  C12Q1/6844

摘要： Systems and methods of identifying nucleobases in a template polynucleotide are disclosed. In one embodiment, such a method may include providing a substrate comprising a plurality of double stranded template polynucleotides in a cluster. Each double stranded template polynucleotide may comprise a first strand and a second strand. The method may further include contacting the plurality of double stranded template polynucleotides with first primers which bind to the first strand and second primers which bind to the second strand. The method may further include extending the first primers and the second primers by contacting the cluster with labeled nucleobases to form first labeled primers and second labeled primers. The method may further include stimulating light emissions from the first and second labeled primers, wherein an amplitude of the signal generated by the first labeled primers is greater than an amplitude of the signal generated by the second labeled primers. The method may further include identifying the labeled nucleobases added to the first primers and the second primers based on the amplitude of the signal generated by the labeled nucleobases.

公开(公告)号：US20230295718A1

公开(公告)日：2023-09-21

申请号：US18045032

申请日：2022-10-07

申请人： Comell University

发明人： FRANCIS BARANY ,  John William Efcavitch

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6855

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12N15/1068 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6855

摘要： The present invention relates to a method for the highly specific, targeted capture of regions of human genomes and transcriptomes from the blood, i.e. from cell free circulating DNA, exosomes, microRNA, circulating tumor cells, or total blood cells, to allow for the highly sensitive detection of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using combined nuclease, ligation, polymerase, and massively parallel sequencing reactions. The method generates a collection of different circular chimeric single-stranded nucleic acid constructs, suitable for sequencing on multiple platforms. In some embodiments, each construct of the collection comprised a first single stranded segment of original genomic DNA from a host organism and a second single stranded synthetic nucleic acid segment that is linked to the first single stranded segment and comprises a nucleotide sequence that is exogenous to the host organism. These chimeric constructs are suitable for identifying and enumerating mutations, copy changes, translocations, and methylation changes. In other embodiments, input mRNA, lncRNA, or miRNA is used to generate circular DNA products that reflect the presence and copy number of specific mRNA's, lncRNA's splice-site variants, translocations, and miRNA.

公开(公告)号：US20230295702A1

公开(公告)日：2023-09-21

申请号：US18147149

申请日：2022-12-28

申请人： Yuuki Yonekawa ,  Hirotaka Unno ,  Satoshi Nakazawa ,  Yunong Ji ,  Kazusa DNA Research Institute

发明人： Yuuki YONEKAWA ,  Hirotaka UNNO ,  Satoshi NAKAZAWA ,  Yunong JI ,  Sakae ITOGA

IPC分类号： C12Q1/6851 ,  C12Q1/6844

CPC分类号： C12Q1/6851 ,  C12Q1/6844

摘要： A method of determining a limit of detection and a limit of quantitation in a nucleic acid detection test is provided, the method including, by using a container having a specific configuration, adding a negative specimen that does not contain target nucleic acids to a positive control group and adding a reagent used for a nucleic acid detection test to a negative control group and the positive control group to amplify the target nucleic acids, and determining a smallest specific copy number among specific copy numbers with a detection rate of 95% or greater in the positive control group as a limit of detection and determining a smallest specific copy number among specific copy numbers with CVln of 35% or less as a limit of quantitation in a case where the target nucleic acids are not detected in the negative control group.

公开(公告)号：US20230295687A1

公开(公告)日：2023-09-21

申请号：US18139689

申请日：2023-04-26

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Jonathan Mark Boutell ,  Oliver Miller

IPC分类号： C12Q1/6806 ,  C12N9/22 ,  C12Q1/6844 ,  C12Q1/6874

CPC分类号： C12Q1/6806 ,  C12N9/22 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12N2800/80

摘要： The present disclosure is concerned with compositions and methods for reducing the steps used in the generation of monoclonal clusters by combining the enzymes used for linearization and removal of unused surface primers.

公开(公告)号：US20230287505A1

公开(公告)日：2023-09-14

申请号：US17907457

申请日：2021-03-29

申请人： ROPHELBIO CO., LTD.

发明人： Seonghee CHOI ,  Jong-Han KIM ,  Hun Su CHU ,  Won-Jin JEONG

IPC分类号： C12Q1/6886 ,  C12Q1/6844 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  C12Q1/6844 ,  G01N33/57484 ,  G01N2440/14 ,  C12Q2600/158 ,  C12Q2600/106

摘要： DRG2 of the present invention is a predictor of a therapeutic response or prognosis of a cancer patient with respect to an agent for cancer immunotherapy in PD-L1 positive cancer and is a biomarker that can be used in companion diagnosis for determination of the administration of an agent for cancer immunotherapy in PD-L1 positive cancer. By determining whether to administer an agent for cancer immunotherapy through the present invention, the agent for cancer immunotherapy can be selectively administered to a patient who will show a therapeutic response, and thus is expected to treat PD-L1 positive cancer more effectively.

公开(公告)号：US20230287478A1

公开(公告)日：2023-09-14

申请号：US18120024

申请日：2023-03-10

申请人： 10x Genomics, Inc.

发明人： Felice Alessio BAVA

IPC分类号： C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6874

CPC分类号： C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6874

摘要： The present disclosure relates in some aspects to methods, probes, and kits for detection of a target analyte in a sample. In some embodiments, disclosed herein are methods in which a biological sample is contacted with a pre-formed detectable probe comprising a concatemeric region, e.g., a rolling circle amplification (RCA) product, comprising multiple copies of a unit sequence. Also disclosed herein are related probes and kits.